Can any one please help me? I have to make a stock solution as well as working solution. For my assay, working solution of 0.01U/ml is needed. I've attached the specifications of enzyme which are mentioned on packaging.
Mark Ian Fowler
- The enzyme is supplied in a 0.5mg/ml solution.
- The actual number of units per mg should be printed on the vial or on its Certificates of Analysis. I'll use 20 units/mg as an example.
- 0.5mg/ml = 10units/ml
- Therefore you will need to dilute the stock solution 1 in 1000 to get a 0.01U/ml working stock.
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Microbiology
- Assays
More Divya Bhardwaj's questions See All
Similar questions and discussions