34 Questions 32 Answers 0 Followers
Questions related from Zonghan Gan
Hi! I use UV and photoinitiator to gel my PEG-acrylate hydrogel. But I observe that when the temperature is lower (at night during winter), it's very easy to get pre-gelled before molding, even...
02 March 2023 9,812 1 View
Hi! It is known that in wound healing and foreign body reaction, M1 macrophage is responsible for recruiting fibroblast and cause fibrosis. However, studies have also shown that forcibly polarize...
26 October 2022 1,567 0 View
Hi! It is known that different integrin has impacting macrophage's polarization, eg. ↑integrin b5, or ↓integrin b3, a3, a6 will shift the macrophage to more M2 behaving. Any more brilliant...
25 October 2022 9,550 0 View
Hi! I am looking for a summary/review of overall different integrin isoforms' affinity to different ECM ligand isoforms。 eg. integrin a7b1 has high affinity to Laminin a2, while a3b1 and a6b1 has...
24 October 2022 7,873 2 View
Hi! As in question, the organoid contains artificial hydrogel thus hard to extract single cell solution. Maybe we should start from immuno-staining? Stain surface markers like CD 163, or using...
09 October 2022 8,955 0 View
Is there a review paper in arthritis just like "Hallmarks of cancer" in Cancer research?
25 August 2022 8,864 2 View
Hi! I am trying to input bead into microfluidics. Thus using a syringe with a magnetic stir bar in it. however, beads often aggregate and clog in the plastic hat that connect syringe and needle,...
17 October 2021 659 2 View
Hi! Electrophoresis 2005, 26, 501–510 reported that biotin-streptavidin can be fully, reversibly disassociated in de-ionised water at elevated temperature @ ~70℃. I tried to repeat with 20um...
31 August 2021 10,004 2 View
Hi! A special primer has to be used with only 19 bp poly dT (Tm 44.9) instead of 30 bp in original study (Tm 55.2). Will this induce failure of mRNA capture? Will this cause RNA detachment in...
07 August 2021 5,500 3 View
Hi! I recently become quite interested in mitochondria research and trying to capture a landscape. What is the most focused phenotypes for mitochondria (including swelling, electron chain...
08 July 2021 2,397 1 View
Hi! We are using microfluidics to fabricate gel beads. Though they have a central mean, the distribution presents a quite discrete distribution. However for our application we need to strictly...
18 May 2021 7,073 3 View
I am planning to have endonuclease Ⅳ and Bst/Taq work in the same buffer, but definitely in different Temperature, i.e, the system will be passing first 37℃ then 60/95℃. Can I simply put...
14 May 2021 3,212 3 View
I have a DNA sample which in previous step induced Brij 35. can I directly input this sample to PCR reaction? Will existence of Brij 35 interrupt the taq enzyme and the PCR reaction?
13 May 2021 316 3 View
Among many papers a convenient method was used to denature DNA, which is to use the solution of NaOH+Brij. The role of NaOH is easy to understand, but what is the effect of Brij 35...
13 May 2021 3,594 5 View
It is well known that Fpg and Endonuclease IV can cleave AP site in double strand DNA. But can they cleave AP (Apurinic/apyrimidinic) site in single strand DNA?
13 May 2021 3,911 2 View
Same question in https://forum.image.sc/t/how-to-automatically-measure-transparent-particle-diameter-with-imagej/51754?u=imagejan Hi! I have got some images of transparent gel bead. Because it...
21 April 2021 3,685 3 View
Hi! I am trying to pack one bead per drop using microfluid. I previously assumed that just adjust bead concentration in accordance to how many drop per minute. Yet this route failed. I think I...
04 March 2021 8,340 2 View
HI! I am trying to pack 1 customised hydrogel bead in 1 drop using microfluid, yet the bead ends up outside the drop. The customised bead (diameter 32um) also made from microfluid. The packing...
04 March 2021 7,358 1 View
Hi! I noticed that mostly the single cell assay use AMPure beads (magnetic) instead of purifying column. But both function through a DNA affinity coating, just on surface in beads and on membrane...
28 January 2021 9,214 5 View
Hi! We are generating hydrogel beads(50-60um) using microfluidics as single-cell beads. To simply all the experiments (especially the centrifuge), I am looking for a dye. The dye should be stable...
15 January 2021 4,560 8 View
It is known that the Uracil-DNA Glycosylase can remove the basic residue of dU. Thus the DNA chain will further cleave at the point of dU when heating to 95℃ However, is there any method to cleave...
19 December 2020 7,064 3 View
We are designing a probe with hydrogel substrate. Can polyacrylamide survive from 95℃，or is there any other type of hydrogel such as PEG that can survive from this temperature?
19 December 2020 2,742 4 View
Hi! We are aiming to bind streptavidin to polyacrylamide surface. The EDC/NHS method bounced on me as a common method, utilizing the -NH2 of polyacrylamide and -COOH of streptavidin. Is there any...
18 December 2020 8,086 2 View
We are fabricating acrylamide beads as probe substrate. How can we make streptavidin bind to the surface of the acrylamide? Thanks!
10 December 2020 8,300 7 View
Hi! We are designing a genome probe, which need to be cut off the acrydite substrate. Yet the photocleavable spacer is too expensive (＄500 for 50nmol primer, which is just 1 time of assay). Is...
08 December 2020 1,866 2 View
We are designing a nucleotide probe for human gene. We need to then cut off the probe from the substrate. Yet the photocleavable space is tooooo expensive. We are thinking to use an endonuclease....
08 December 2020 5,978 3 View
Hi! After weeks of testing we get control of the drop size, by put oil pump vertical and adjust the oil flow rate. However, we found the drop size becomes crazily deviated when trying to get drop...
29 October 2020 1,181 6 View
Hi! We realise the dripping in our microfluid chip (the + cross). Yet we wonder how can we control our droplet size to ~=70um sphere (the channel is a semicircle in 70um diameter) Can we...
15 October 2020 8,809 8 View
It is suggested that as a measurement of the collagen stability, the denature temperature Td is comprised by both the hydrogen bond from triple helix and an entropic part of the fibril. The...
14 August 2020 5,595 1 View
Some studies point that crosslinking increase the inflammatory reaction of collagen[1-3]. while some other studies suggests that Cross-linking also can be introduced to reduce the antigenicity....
11 August 2020 3,393 3 View
I havd found many researches on fabrication of collagen gel always using soluble and insoluble collagen (same-source) respectively. The only difference is the tellopeptide in soluble collagen is...
11 August 2020 4,610 3 View
Please don't answer because U(T,V) don't have S entropy as argument!!!!!! May I ask a question on thermodynamic? We know that U(V,T) (caloric eq. of state) and S(P,V) (thermodynamic eq of state)...
01 May 2020 7,196 1 View
Hi! We are doing studies about tissue engineering using hybrid scaffold composed of PCL and collagen. However get stuck in RNA extraction for gene expression analysis. Some study suggest using the...
19 July 2019 927 3 View
Finding books or other materials systematically explain the structure of protein and how they interact with each other(the forces, the chemical properties). It is even better if it also cover...
11 July 2019 6,477 2 View
