Hi!
We are aiming to bind streptavidin to polyacrylamide surface. The EDC/NHS method bounced on me as a common method, utilizing the -NH2 of polyacrylamide and -COOH of streptavidin. Is there any unconsidered factors that may block this route? If EDC/NHS is viable to immoblise streptavidin to acrylamide, how should the reaction condition be set? Consider the static charge of polyacrylamide and streptavidin ( isoelectric point = 6), which pH is optimistic? Should we use a 2 step EDC/NHS xlk or 1 step?
Thanks!
The NH2 group of acrylamide is not an amine, but an amide, which is usually not suitable for EDC/NHS chemistry. It very much depends on your system, what to do next. If you are synthesizing your polyacrylamide yourself, you have plenty of options to introduce more suitable monomers. Another approach might be the simple, non-covalent adsorption of streptavidin. From my point of view, you only should consider more complex protocols, if the direct adsorption fails.
Thanks all! Alaa A. A Aljabali
Michael G. Weller Direct absorption is definitely an option. The streptavidin-acrylamide is too expensive for our purpose. Is there any other option to covalently bind streptavidin to polyacrylamide or add other monomer such as?- Badges
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