Will Brij-35 in the system interrupt PCR and taq enzyme?

More Zonghan Gan's questions See All

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

How to add viscosity into a hyperelastic material such as Mooney-Rivlin rubber in LS-DYNA?

Hi, I am working on a head FE model and want to try different material types on brain. I read some papers that use Mooney-Rivlin rubber model and wondering if i can add viscosity into it. Is it...

24 July 2024 5,908 0 View

Is absolute max principal strain the same to max principal strain in HyperView?

Hi, I run an FE model on LS-DYNA solver, and concerning about the max principal strain contour result in HyperView. When I choose the result type as Strain(t)-Absolute Max Principal the contour...

19 June 2024 7,577 1 View

How to identify the correct end point from a titration curve?

Hi, I am running a titration of QAC with sodium tetraphylborate using autotitrator from Metrohm. The titrator identifies multiple end point with different ERC value. Would like to know which EP...

17 April 2024 545 1 View

Which cell line that is neither expressing iNOS Nor CD163?

Hi! I am staining iNOS and CD163 in my cells so I am looking for a minus control, expressing neither iNOS nor CD163. But the cell familiar in our lab including panc-1 and 293T seems at least...

04 April 2024 7,855 1 View

Why should we incubate ABTS stock solution for 16 hours?

ABTS stock preparation was prepared by adding ABTS and potassium persulphate.Can we use it immediately?If Cannot why?

22 December 2023 8,444 0 View

Can anyone recommend some tissue-specific gene (leaf, fruit, root) for tomato?

I am planning to design a lab practical to extract RNA from different parts of tomato, namely leaf, fruit and root, followed by expression analysis. Can anyone recommend some tissue specific...

01 November 2023 3,523 0 View

Why the Navier-Stokes equations exhibit material-frame indifference for two-dimensional flow?

According to the book written by Pope, the vorticity equations exhibit material-frame indifference when the flow is two-dimensional and the rotations of the frame are steady. But how can we judge...

15 May 2023 4,791 3 View

Why cold makes my PEG acrylate hydrogel gel faster?

Hi! I use UV and photoinitiator to gel my PEG-acrylate hydrogel. But I observe that when the temperature is lower (at night during winter), it's very easy to get pre-gelled before molding, even...

01 March 2023 9,886 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View