I havd found many researches on fabrication of collagen gel always using soluble and insoluble collagen (same-source) respectively. The only difference is the tellopeptide in soluble collagen is removed. After crosslinking there wouldn’t be any solubility difference. The reason of using both col is mostly unstated. Could it be because the hydrophobic bond of tellopeptide in insoluble collagen influence the bundle size during gelation?
This early 1968 JBC paper may give you some insight. "A Comparison of Glycopeptides Derived from Soluble and Insoluble Collagens* ".
According to this paper the two fractions of connective tissue are the low and high mol weight fractions. The later is heterogeneous and has the poly lysine cross links that combined makes it insoluble. Also the two fractions changes with age and most likely several other factors. The utility of the two will most likely depend on what kind of study you want. I have used commercial soluble collagen and extracted whole collagen and used them for cell substrates. Their are literature that state whole collagen has marked advantages as it will be closer to the biological composition. But since the insoluble component is heterogeneous, soluble component studies are are better controlled experiments and thus expected to have better reproducibility.
Dear all, the following document discusses the main structural pecularities of soluble and insoluble fractions of collagen. My Regards
https://www.nature.com/articles/217168a0
I guess, it is some like soluble starch and insoluble starch. Both have the same chemical structures, but with different configuration.
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