Same question in
https://forum.image.sc/t/how-to-automatically-measure-transparent-particle-diameter-with-imagej/51754?u=imagejan
Hi!
I have got some images of transparent gel bead. Because it is transparent, I cannot turn it into masked Black&white image using "8-bit->threshold->set measurements (Feret's Diameter)".
1. is there another way to obtain the outline and turn it into a solid black&white image for diameter analysis?
2. Alternatively, is there a macro script/plugin that are designed for such transparent praticle?
3. in some previous images, the gel beads are overlap with each other. How to process the overlap images for the diameter analysis in batch?
Thank you!
the image scale is 543.75 pixel=100um
You asked the same question here on the image.sc forum: https://forum.image.sc/t/how-to-automatically-measure-transparent-particle-diameter-with-imagej/51754?u=imagejan
... and received an answer already. Please always disclose when cross-posting in different channels, to allow others to follow the discussion. Let's keep the discussion on the image.sc forum, the primary channel for image analysis help.
Jan Eglinger Sorry I did not know this before. I add a disclose. Thank you!
Dear Zonghan Gan
Try to enter a single value in Size and particles smaller than that value are ignored.
Hope this helps.
Regards
Qamar Ul Islam
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