What is the best way to characterize macrophage M1/M2 polarization in organoid?

09 October 2022 0 9K Report

Hi!

As in question, the organoid contains artificial hydrogel thus hard to extract single cell solution. Maybe we should start from immuno-staining?

Stain surface markers like CD 163, or using JC-10 (JC-1) to stain mitochondria potential? Will JC-10 as small molecule penetrate the tissue better or worse, than antibodies?

Also at the first stage we just want to tell between M1/M2, not the M2 sub groups. If characterizing surface markers, what CD set should we choose (eg. CD80 for M1 and CD163 for M2?)?

Thanks!

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