Hi!
I am staining iNOS and CD163 in my cells so I am looking for a minus control, expressing neither iNOS nor CD163. But the cell familiar in our lab including panc-1 and 293T seems at least expressing 1 of them. Which cell line is known to not having observable level of these 2 proteins?
Thanks!
A cell line that does not express both iNOS (inducible nitric oxide synthase) and CD163 (cluster of differentiation 163) could be a cell line derived from a tissue or cell type that does not typically express these markers under normal conditions.For example, many cancer cell lines, such as HeLa cells or MCF-7 cells, may not express iNOS or CD163 unless they are specifically induced to do so under experimental conditions. Additionally, certain immortalized cell lines derived from non-immune tissues, such as fibroblasts or epithelial cells, may not express these markers.However, it's important to note that the expression of iNOS and CD163 can vary depending on the experimental conditions and the specific context in which the cells are studied. Therefore, it's always a good idea to confirm the expression profile of a cell line using experimental techniques such as immunostaining, Western blotting, or flow cytometry.