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Questions related from Vinay Kant
We have evaluated the wound healing potentials of a compound at a particular concentration in ointment form in laboratory animals. Now, we are preparing different types of topical formulations...
15 January 2020 3,194 6 View
I am testing some compounds for wound healing in rats (rodent) and want to further extend the research for the drug development for wound healing in animals/human. So, please tell me the different...
31 March 2019 8,503 3 View
We are not able to completely dissolve the lecithin crude form (P5638 Sigma , L-α-Phosphatidylcholine from soybean, Type II-S, 14-29% choline basis) in ethanol for the preparation of...
15 December 2018 2,523 0 View
I am interested to assess the skin permeation potential of some formulation prepared for topical use for wound healing or other problem of skin. For this study, I want to use franz diffusion cell...
21 April 2018 2,826 1 View
I want to conduct the experiment in poultry or other species for the evaluation of growth promoter potential of herbal or polyherbal product. Please suggest me the appropriate approach.
10 March 2017 700 2 View
I want to enhance the dermal as well as systemic delivery of my compounds for the acceleration of wound healing by nanotechnology. But, I am not sure about the right approach. So, please tell me...
08 March 2017 9,385 6 View
I want to make excision wound model in rats for the study of drugs for healing potential. So, i want to know that is simple open excision wound model is better than stented wound model in rats or...
22 June 2016 4,168 4 View
I want to apply quercetin topically on wounds of animals, but quercetin is not soluble in water. So, please suggest me some appropriate vehicles of its topical application on wounds.
01 May 2016 7,499 5 View
Different hormones are produced by hypothalamus, anterior pituitary and other endocrine glands. Somewhere it is written that these are tropin hormone but at some places tropic word is used. So, I...
20 February 2016 7,603 6 View
I want to evaluate the evaluate the switching of macrophages from M1 to M2 phenotype during cutaneous wound healing. So, please tell me the specific markers of M1 and M2 macrophages for...
03 October 2015 7,544 11 View
I would like to test my compound for the antibacterial potential, so I would like to use the appropriate agar and broth for the better results. I am confused between nutrient agar, muller hinton...
17 May 2015 900 54 View
I want to test the compound for antibacterial activity and i want to use one or two standard antibiotic. So, please tell me the ideal one which is used by most of the antibacterial testing...
17 May 2015 10,112 14 View
I want to measure the MIC and MBC for my compound. Which method is better as both agar and broth method can be used for MIC. Please tell the best method and which type of agar or broth should be...
17 May 2015 9,477 25 View
Blotting the same membrane for two different protein saves the time and cost. There are many ways of doing this either with stripping or without stripping. Which is the better approach and can we...
13 January 2015 474 2 View
I want to apply the quercetin topically on wounds, so I want to know the most suitable vehicle for its application on wounds.
13 January 2015 8,160 6 View
I want to assess the effect of quercetin on wound healing, so I want know the dose of quercetin for topical application. Is there any literature of dose of quercetin for topical application or...
13 January 2015 7,617 1 View
The proteins in the cell are located at many places. Like the protein can be Cytoplasmic soluble or cytoskeletal bound or Membrane bound or Nuclear or Mitochondria or in the whole cell. I want to...
02 November 2014 9,602 2 View
I am doing estimation of different antioxidant enzymes and evaluating the oxidative stress in adrenal gland in different toxicities. So, can anyone tell me the normal levels of glutathione...
28 August 2014 4,928 4 View
Determination of protein concentration is very important befor loading the samples in wells. So, can you please tell me the most effective and easy method by which protein can be quantified in...
26 August 2014 7,784 11 View
I want to purchase Trizol or Ribozol for RNA isolation from skin tissues. Can you suggest a company that provides good RNA purity. What is your opinion on Trizol from Tacara or invitrogen?
22 August 2014 2,240 5 View
I want to purchase the prestained protein marker for the WB for my proteins of interest (size of my proteins vary from 12-100 kD). So, can you suggest me the company which provide the good quality...
20 August 2014 6,763 6 View
I want to purchase the PCR Master Mix for my research. Can you suggest the type of PCR master mixture I should use and which company/brand provides it at suitable cost. Does anyone have experience...
20 August 2014 9,387 7 View
I want to purchase the monoclonal antibodies of VEGF, TGF-beta1, α-SMA and β-actin against rats for my research. Can you suggest the company/brand whose antibodies provide good results? I have...
20 August 2014 7,313 3 View
Selection of membrane is very important for expression of proteins with good quality in WB. So I want to know the best membrane and its supplier so that good results can be obtained.
16 August 2014 3,204 9 View
I want to estimate the cholesterol levels in different tissues like adrenal gland, ovary, liver etc. Can anyone provide me the method of estimation of cholesterol in these tissues.
16 July 2014 671 5 View
There are two approaches by which we can use the water insoluble quercetin in efficient way for topical use. 1) nano quercetin incorporated in chitosan. 2) quercetin loaded in nanoparticle of...
04 June 2014 5,418 1 View
Sometime we test a drug in vitro for evaluating its anti-apoptotic effect or antibacterial effect and that basis we can calculate the IC50. Now, can we use this information in calculating the...
29 April 2014 6,131 7 View
Aceclofenac is metabolized into various metabolites and Diclofenac is one of them and this Diclofenac has been banned for its use in cattle due to vulture mortality in South Asia. So, should we...
30 January 2014 3,643 5 View
Nitrofurazon is more active at acidic pH, but it is used in combination with urea for prevention of uterine infections. The urea increases the pH of the environment, so I want to know how the...
18 December 2013 9,499 0 View
I want to now the exact method/approach of selection of a drug for wound healing experiments. Sometime the dose used in the published paper is used and sometime some pilot experiments are carried...
01 December 2013 9,157 1 View
In diabetes vascular occlusion is present, which interfere in the healing process and delay the wound healing. I want to to know that why which method (any special staining protocol) it is...
22 November 2013 9,331 0 View
Streptozotocin injection produces diabetes within two days, but this is considered as acute diabetes. I want to know that after how many days these streptozotocin injected rats can be considered...
22 November 2013 2,853 2 View
Remodeling is one of the phases of wound healing, which persists for the longest period as compared to other phases. So, to accelerate wound healing I want to target this phase. Can anyone please...
08 November 2013 5,812 4 View
In wound healing, we observed that the contraction of the wound cause significant reduction in the size of the wound. On the basis of this, some researchers have suggested that the agent/drugs...
15 October 2013 4,297 5 View
During my research in cutaneous wound healing in diabetics, I observed that the GAP-43 positive nerve fibers increased with the increase in the blood vessels number. Also, the distribution of...
04 October 2013 7,901 8 View
Increased formation of fatty connective tissue in the healing tissue of cutaneous wound in diabetic rats has shown decreased formation of granulation tissue in streptozotocin induced diabetic rats.
17 August 2013 5,693 1 View
