What are the locations of beta-actin, VEGF and TGF-beta1 in skin or granulation tissue of rat?

More Vinay Kant's questions See All

How to calculate chemical quantity required when relative molar composition is given ?

Relative molar composition of 1.0 TEOS : 0.2 CTACl : 0.0026–0.017 LS277 dye : 10.4 TEA : 142 H2O was used.

04 April 2024 6,770 1 View

Scoliosis Research Society srs22r questionaire in german ?

Looking for the srs22r questionnaire for evaluation of scoliosis patients. The study group in Tübingen (Germany) - have validated and published this. Can anyone kindly send me the srs22r score...

20 March 2024 8,696 0 View

GPPS/Glass fiber composite?

I am preparing general purpose polystyrene (GPPS) glass fiber composite. I want to know what coupling agent can be used and what glass fiber grade would be suitable for GPPS

17 March 2024 8,465 1 View

User Graph Traversal with weighted edges: Should we consider a multiplication of confidence scores in finding related nodes?

Hello! I have the following heterogenous graph - One of the vertices represent users and weighted edges between these user vertices represent the confidence we have in those users being the...

25 February 2024 9,930 0 View

Conductive Ink using CNT/ graphene/ nanoparticles?

Hii, Can anyone please suggest articles for preparation of conductive ink using CNTs/graphene/ nanoparticles, etc.?

22 February 2024 7,694 2 View

Is the drug adsorbed or absorbed in nanoparticle during or after loading the nanoparticle with the drug?

How does it depend on the nature or class of nanoparticles?

02 February 2024 4,015 2 View

Can any one help me on military leadership?

Dear colleague, I am looking for assessment tools for Military Leadership Style and Competences. However, I am finding difficult to get information on the same. Can anyone help...

02 January 2024 1,584 4 View

Does Exploratory Factory essential for test development?

It is a established practice in psychology and management that in a test development process exploratory factor analysis is essential to determine/define the factors/construct of test....

28 December 2023 6,165 8 View

Are we overloading our research in social sciences?

In recent past there's a increasing trends in the western world publishing houses? They are more focused on language than the methodological aspect and a huge amount is being asked for such...

13 December 2023 4,323 0 View

How to prepare H2O2 standard graph at 390nm from 30% H2O2?

H2O2 content from plant leaf under salt stress

10 November 2023 5,718 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Dr. Mohammad Iqbal Yatoo

TGF-B1 also in ECM

James Andrew Thompson

TGF-B1 will be found in the ECM in its latent form, associated with latency associated peptide (LAP) and one of the latent TGF-B binding proteins (LTBP). The LTBP is able to anchor it to the ECM until it is activated by proteases or integrins etc.