How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

What needs to be taken care of while fitting a ferromagnetic resonance signal? In context of the amplitude, Real part and imaginary part of the FMR?

I am using a lock in amplifier detection technique along with an Helmholtz coil. I am measuring the real and imaginary part of the FMR signal. Which signal (Amplitude, Real or imaginary) should be...

16 August 2020 1,233 6 View

Where can I find a detailed database with info on how many people did COVID 19 patient meet before being symptomatic?

We are building a mathematical model for COIVD 19. We are looking for data with Patient ID, Age, Gender, Travel from and to, Date of arrival, a number of people the patient met after arrival,...

29 March 2020 7,954 4 View

How to plot data in 10X10 matrix in which all the diagonal elements are 0 and the matrix is symmetric about the diagonal?

I have a data of comparison of 10 proteins with each other under two parameters i.e 100 entries in the shells of two 10X10 matrix in excell sheet. I want to see whether both of these data sets...

02 March 2020 1,593 5 View

Limitation of Clausius-Mossotti relation??

what is the limitation for Clausius-Mossotti relation?? is that applicable for all the crystal structure??. If the material is an organic single crystal, how well this suits for it

31 January 2020 8,571 3 View

Does it require testing each topical formulation of our drug in laboratory animals, if drug has showed potential at some concentrations?

We have evaluated the wound healing potentials of a compound at a particular concentration in ointment form in laboratory animals. Now, we are preparing different types of topical formulations...

14 January 2020 2,893 6 View

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09 December 2019 8,735 11 View

Problem in downloading particular ZINC database file, suggestions?

Can anybody provide me with underneath Zinc database (.sdf format) https://zinc.docking.org/substances/subsets/biogenic/ or suggest me how to download this database. Although, I have tried many...

27 November 2019 7,112 8 View

Looking for a simple protocol of DNA Extraction from plant leaves? Suggestions please. Thanks in Advance?

Tried various protocols none is working. Generally getting DNA contaminated with phenols and proteins.

09 October 2019 8,548 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View