Which company/brand provides good quality Trizol or Ribozol for RNA isolation from skin tissues?

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How to calculate chemical quantity required when relative molar composition is given ?

Relative molar composition of 1.0 TEOS : 0.2 CTACl : 0.0026–0.017 LS277 dye : 10.4 TEA : 142 H2O was used.

04 April 2024 6,770 1 View

Scoliosis Research Society srs22r questionaire in german ?

Looking for the srs22r questionnaire for evaluation of scoliosis patients. The study group in Tübingen (Germany) - have validated and published this. Can anyone kindly send me the srs22r score...

20 March 2024 8,696 0 View

GPPS/Glass fiber composite?

I am preparing general purpose polystyrene (GPPS) glass fiber composite. I want to know what coupling agent can be used and what glass fiber grade would be suitable for GPPS

17 March 2024 8,465 1 View

User Graph Traversal with weighted edges: Should we consider a multiplication of confidence scores in finding related nodes?

Hello! I have the following heterogenous graph - One of the vertices represent users and weighted edges between these user vertices represent the confidence we have in those users being the...

25 February 2024 9,930 0 View

Conductive Ink using CNT/ graphene/ nanoparticles?

Hii, Can anyone please suggest articles for preparation of conductive ink using CNTs/graphene/ nanoparticles, etc.?

22 February 2024 7,694 2 View

Is the drug adsorbed or absorbed in nanoparticle during or after loading the nanoparticle with the drug?

How does it depend on the nature or class of nanoparticles?

02 February 2024 4,015 2 View

Can any one help me on military leadership?

Dear colleague, I am looking for assessment tools for Military Leadership Style and Competences. However, I am finding difficult to get information on the same. Can anyone help...

02 January 2024 1,584 4 View

Does Exploratory Factory essential for test development?

It is a established practice in psychology and management that in a test development process exploratory factor analysis is essential to determine/define the factors/construct of test....

28 December 2023 6,165 8 View

Are we overloading our research in social sciences?

In recent past there's a increasing trends in the western world publishing houses? They are more focused on language than the methodological aspect and a huge amount is being asked for such...

13 December 2023 4,323 0 View

How to prepare H2O2 standard graph at 390nm from 30% H2O2?

H2O2 content from plant leaf under salt stress

10 November 2023 5,718 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Utkarsh Reddy

you can use TrI reagent from Sigma too...used for Animal tissues ...No issues

James John

I use Sigma Trizma and it works well for me

Rebecca Ivy Clark

Invitrogen's TRIzol works great for me too.

Breanna Symmes

Ambion---now under "LifeTechnologies."

Matteo Da Ros

I use TRIsure from Biohit, it is exactly the same as TRIzol and TRIreagent, but cheaper!