How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

What needs to be taken care of while fitting a ferromagnetic resonance signal? In context of the amplitude, Real part and imaginary part of the FMR?

I am using a lock in amplifier detection technique along with an Helmholtz coil. I am measuring the real and imaginary part of the FMR signal. Which signal (Amplitude, Real or imaginary) should be...

16 August 2020 1,233 6 View

Where can I find a detailed database with info on how many people did COVID 19 patient meet before being symptomatic?

We are building a mathematical model for COIVD 19. We are looking for data with Patient ID, Age, Gender, Travel from and to, Date of arrival, a number of people the patient met after arrival,...

29 March 2020 7,954 4 View

How to plot data in 10X10 matrix in which all the diagonal elements are 0 and the matrix is symmetric about the diagonal?

I have a data of comparison of 10 proteins with each other under two parameters i.e 100 entries in the shells of two 10X10 matrix in excell sheet. I want to see whether both of these data sets...

02 March 2020 1,593 5 View

Limitation of Clausius-Mossotti relation??

what is the limitation for Clausius-Mossotti relation?? is that applicable for all the crystal structure??. If the material is an organic single crystal, how well this suits for it

31 January 2020 8,571 3 View

Does it require testing each topical formulation of our drug in laboratory animals, if drug has showed potential at some concentrations?

We have evaluated the wound healing potentials of a compound at a particular concentration in ointment form in laboratory animals. Now, we are preparing different types of topical formulations...

14 January 2020 2,893 6 View

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09 December 2019 8,735 11 View

Problem in downloading particular ZINC database file, suggestions?

Can anybody provide me with underneath Zinc database (.sdf format) https://zinc.docking.org/substances/subsets/biogenic/ or suggest me how to download this database. Although, I have tried many...

27 November 2019 7,112 8 View

Looking for a simple protocol of DNA Extraction from plant leaves? Suggestions please. Thanks in Advance?

Tried various protocols none is working. Generally getting DNA contaminated with phenols and proteins.

09 October 2019 8,548 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View