Vinay Kant

28 Questions 27 Answers 0 Followers

Questions related from Vinay Kant

Does it require testing each topical formulation of our drug in laboratory animals, if drug has showed potential at some concentrations?

We have evaluated the wound healing potentials of a compound at a particular concentration in ointment form in laboratory animals. Now, we are preparing different types of topical formulations...

15 January 2020 3,089 6 View

What are the different Nonclinical Pharmacological/Toxicological studies required for the development of Topical Drugs for wound healing?

I am testing some compounds for wound healing in rats (rodent) and want to further extend the research for the drug development for wound healing in animals/human. So, please tell me the different...

31 March 2019 8,410 3 View

How to use lecithin crude form (P5638 Sigma, L-α-Phosphatidylcholine from soybean, 14-29% choline basis) for preparing nanoformulation of flavonids?

We are not able to completely dissolve the lecithin crude form (P5638 Sigma , L-α-Phosphatidylcholine from soybean, Type II-S, 14-29% choline basis) in ethanol for the preparation of...

15 December 2018 2,458 0 View

Which supplier/company provides the good quality franz diffusion cell for in vitro skin permeation study?

I am interested to assess the skin permeation potential of some formulation prepared for topical use for wound healing or other problem of skin. For this study, I want to use franz diffusion cell...

21 April 2018 2,787 1 View

How to standardize the dose of any herbal or polyherbal product in poultry or other species for growth promoter action?

I want to conduct the experiment in poultry or other species for the evaluation of growth promoter potential of herbal or polyherbal product. Please suggest me the appropriate approach.

10 March 2017 649 2 View

Which is the appropriate vehicle for topical application of querectin on wounds?

I want to apply quercetin topically on wounds of animals, but quercetin is not soluble in water. So, please suggest me some appropriate vehicles of its topical application on wounds.

01 May 2016 7,463 5 View

What is the difference between tropin and tropic hormones?

Different hormones are produced by hypothalamus, anterior pituitary and other endocrine glands. Somewhere it is written that these are tropin hormone but at some places tropic word is used. So, I...

20 February 2016 7,511 6 View

Which are the specific markers of M1 and M2 macrophages by immunohistochemistry?

I want to evaluate the evaluate the switching of macrophages from M1 to M2 phenotype during cutaneous wound healing. So, please tell me the specific markers of M1 and M2 macrophages for...

03 October 2015 7,487 11 View

Which is the ideal agar or broth for testing the antibacterial activity of the compound for Staphylococcus aureus and E. coli?

I would like to test my compound for the antibacterial potential, so I would like to use the appropriate agar and broth for the better results. I am confused between nutrient agar, muller hinton...

17 May 2015 802 54 View

Which is the ideal antibiotic(s) as a standard for testing the antibacterial activity of the compound for Staphylococcus aureus and E.coli?

I want to test the compound for antibacterial activity and i want to use one or two standard antibiotic. So, please tell me the ideal one which is used by most of the antibacterial testing...

17 May 2015 9,994 14 View

What is difference between minimum inhibitory concentration (MIC) and MBC and How to calculate these two for any compound or drug?

I want to measure the MIC and MBC for my compound. Which method is better as both agar and broth method can be used for MIC. Please tell the best method and which type of agar or broth should be...

17 May 2015 9,330 25 View

Can we blot the membrane for two different proteins by using specific monoclonal antibodies from the same origin/species after mixing both together?

Blotting the same membrane for two different protein saves the time and cost. There are many ways of doing this either with stripping or without stripping. Which is the better approach and can we...

13 January 2015 406 2 View

Which is the most suitable vehicle for topical application of quercetin hydrate?

I want to apply the quercetin topically on wounds, so I want to know the most suitable vehicle for its application on wounds.

13 January 2015 8,109 6 View

How do you select the dose of quercetin for topical application on wounds?

I want to assess the effect of quercetin on wound healing, so I want know the dose of quercetin for topical application. Is there any literature of dose of quercetin for topical application or...

13 January 2015 7,564 1 View

What are the levels of glutathione peroxidase, MDA, in adrenal gland of rats?

I am doing estimation of different antioxidant enzymes and evaluating the oxidative stress in adrenal gland in different toxicities. So, can anyone tell me the normal levels of glutathione...

28 August 2014 4,884 4 View

Which method is good to quantify the protein concentration tissue lysate before loading in well for western blotting?

Determination of protein concentration is very important befor loading the samples in wells. So, can you please tell me the most effective and easy method by which protein can be quantified in...

26 August 2014 7,726 11 View

Which company/brand provides good quality Trizol or Ribozol for RNA isolation from skin tissues?

I want to purchase Trizol or Ribozol for RNA isolation from skin tissues. Can you suggest a company that provides good RNA purity. What is your opinion on Trizol from Tacara or invitrogen?

22 August 2014 2,185 5 View

Which company/brand provide the good quality prestained protein marker for the western blottiing ?

I want to purchase the prestained protein marker for the WB for my proteins of interest (size of my proteins vary from 12-100 kD). So, can you suggest me the company which provide the good quality...

20 August 2014 6,721 6 View

Which company/brand provides good quality and economic PCR master mix?

I want to purchase the PCR Master Mix for my research. Can you suggest the type of PCR master mixture I should use and which company/brand provides it at suitable cost. Does anyone have experience...

20 August 2014 9,337 7 View

Where can I get good and economic monoclonal antibodies for VEGF, TGF-beta1, α-SMA and β-actin against rats?

I want to purchase the monoclonal antibodies of VEGF, TGF-beta1, α-SMA and β-actin against rats for my research. Can you suggest the company/brand whose antibodies provide good results? I have...

20 August 2014 7,269 3 View

Which is the best membrane for transfering the proteins in western blotting and company's membrane give good results?

Selection of membrane is very important for expression of proteins with good quality in WB. So I want to know the best membrane and its supplier so that good results can be obtained.

16 August 2014 3,148 9 View

What is the method of estimation of cholesterol levels in tissues?

I want to estimate the cholesterol levels in different tissues like adrenal gland, ovary, liver etc. Can anyone provide me the method of estimation of cholesterol in these tissues.

16 July 2014 618 5 View

What is the best approach for the topical delivery of quercetin?

There are two approaches by which we can use the water insoluble quercetin in efficient way for topical use. 1) nano quercetin incorporated in chitosan. 2) quercetin loaded in nanoparticle of...

04 June 2014 5,370 1 View

Can we determine the LD50 of any drug for rats on the basis of IC50 values of the drug in cell culture?

Sometime we test a drug in vitro for evaluating its anti-apoptotic effect or antibacterial effect and that basis we can calculate the IC50. Now, can we use this information in calculating the...

29 April 2014 6,066 7 View

Should Aceclofenc be banned due to potential threat to vultures?

Aceclofenac is metabolized into various metabolites and Diclofenac is one of them and this Diclofenac has been banned for its use in cattle due to vulture mortality in South Asia. So, should we...

30 January 2014 3,605 5 View

Which are the reliable and important parameters to determine the good remodeling in wound healing.?

Remodeling is one of the phases of wound healing, which persists for the longest period as compared to other phases. So, to accelerate wound healing I want to target this phase. Can anyone please...

08 November 2013 5,769 4 View

Is wound contraction reliable parameter in wound healing?

In wound healing, we observed that the contraction of the wound cause significant reduction in the size of the wound. On the basis of this, some researchers have suggested that the agent/drugs...

15 October 2013 4,236 5 View

Is there any association between number of nerve fibers with the increased blood vessels number and their vasodilation in healing cutaneous wounds?

During my research in cutaneous wound healing in diabetics, I observed that the GAP-43 positive nerve fibers increased with the increase in the blood vessels number. Also, the distribution of...

04 October 2013 7,855 8 View