I want to evaluate the evaluate the switching of macrophages from M1 to M2 phenotype during cutaneous wound healing. So, please tell me the specific markers of M1 and M2 macrophages for immunohistochemistry
Hello
in general you can use CD163 ,CD68+ , for more information please look at the links & attachment here
also there are discussion in RG about this issues will help you
Good Luck
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829941/
http://www.ncbi.nlm.nih.gov/pubmed/15491976
https://www.researchgate.net/post/Are_there_any_specific_cell_surface_markers_for_M1_and_M2_macrophages
http://www.atherosclerosis-journal.com/article/S0021-9150(12)00631-4/abstract
https://www.abdserotec.com/macrophage-polarization-minireview.html
https://etd.ohiolink.edu/!etd.send_file?accession=ohiou1357313250&disposition=inline
Hello:
Hoping this information helps, wishing you best luck.
http://www.ncbi.nlm.nih.gov/pubmed/25368618
Regards,
Regarding mouse I would use iNOS and for M2 you could use Mannose receptor or Arginase-1. Regarding Human I would use CD163 and CD68.
INOS I used to be M1 for Mouse;CD163 I used to be M2 for human; CD68 I used to be total macrophages.
I want to differentiate M1 and M2 macrophages of rat wound tissue so want to know the specific marker for them in rat
Hi Vinay,
In that case you could use either CD80 or CCR7 for M1 and CD163 for M2 macrophages. The pan marker for macrophages in rat is CD68.
Hi,
CD206/MR and Arginase-1 have been classically considered M2 and iNOS, IL-1, TNF, have been classically classically considered M1. CD206/Mannose receptor and Arginase-1 protein expression was low but has been done by IHC. However, some M1 macrophages can up-regulate Arg-1.
We have recently identified novel markers that are exclusive of M1or M2 macrophages, are expressed as protein at high level and work by flow. It would be interesting to see how they work by IHC- we have so far done flow cytometry.
Our strategy: select genes up-regulated during M1 differentiation but down-regulated during M2 differentiation and vice versa. The results of this study and the new markers we have identified, including a new CD38/Egr2-based flow cytometry strategy to distinguish M1 and M2 macrophages, can be found in Jablonski et al. Plus One. 2015 (find link below).The markers described in this paper allowed to perfectly distinguish in vitro M1 and M2 macrophages and also identified inflammatory populations in vivo.
Article Novel Markers to Delineate Murine M1 and M2 Macrophages
According to:
Barros MH, Hauck F, Dreyer JH, Kempkes B, Niedobitek G.Macrophage polarisation: an immunohistochemical approach for identifying M1and M2 macrophages. PLoS One. 2013 Nov 15;8(11):e80908. doi: 10.1371/journal.pone.0080908. eCollection 2013.
Jablonski KA, Amici SA, Webb LM, Ruiz-Rosado Jde D, Popovich PG, Partida-Sanchez S, Guerau-de-Arellano M.
Novel Markers to Delineate Murine M1 and M2 Macrophages. PLoS One. 2015 Dec 23;10(12):e0145342. doi: 10.1371/journal.pone.0145342. eCollection 2015.
CD38 for M1 and IL-10 for M2
CD68 generally for macrophage detection
For mRNA qPCR:
1: Xu L, Yang F, Lin R, Han C, Liu J, Ding Z. Induction of m2 polarization in primary culture liver macrophages from rats with acute pancreatitis. PLoS One. 2014 Sep 26;9(9):e108014. doi: 10.1371/journal.pone.0108014. eCollection 2014. PubMed PMID: 25259888; PubMed Central PMCID: PMC4178066.
Please see this paper where membrane-associated proteins have been identified that can discriminate M1 and M2 macrophages: Article Unique Proteomic Signatures Distinguish Macrophages and Dend...
Please also see our recent study that utilized this information in order to identify macrophage polarity: Article Nanomedicine-driven neuropathic pain relief in a rat model i...
I hope this helps.
Follow up question, can we use TLR4 as a marker for M1 only? or will it show on M2 cells as well?
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