I am doing estimation of different antioxidant enzymes and evaluating the oxidative stress in adrenal gland in different toxicities. So, can anyone tell me the normal levels of glutathione peroxidase and MDA in adrenal gland of rats.
MDA levels you can determine the dosage by simply because all the organs are composed of rich membrane phospholipid but GPx it comes to determining what type or isoform of GPx first
any normal level first it depend on which method you are going too use , types of rats ( wistar / spraug dowley or some other varities ) , laboratory condition of that rats , so please tell me the which method and which rats you are going to use
Dear Arbind,
the rats are wister and if you have the levels of any of these parameters by any method then please tell, we will also do by that method..
thanks
dear Kant
MDA-TBA reactive substances values were expressed as nmol per milligram of tissue protein (nmol/mg protein).
The glutathione peroxidase activity was expressed as unit per mg of tissue
protein (U/mg protein). (Special activity)
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