How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

What needs to be taken care of while fitting a ferromagnetic resonance signal? In context of the amplitude, Real part and imaginary part of the FMR?

I am using a lock in amplifier detection technique along with an Helmholtz coil. I am measuring the real and imaginary part of the FMR signal. Which signal (Amplitude, Real or imaginary) should be...

16 August 2020 1,233 6 View

Where can I find a detailed database with info on how many people did COVID 19 patient meet before being symptomatic?

We are building a mathematical model for COIVD 19. We are looking for data with Patient ID, Age, Gender, Travel from and to, Date of arrival, a number of people the patient met after arrival,...

29 March 2020 7,954 4 View

How to plot data in 10X10 matrix in which all the diagonal elements are 0 and the matrix is symmetric about the diagonal?

I have a data of comparison of 10 proteins with each other under two parameters i.e 100 entries in the shells of two 10X10 matrix in excell sheet. I want to see whether both of these data sets...

02 March 2020 1,593 5 View

Limitation of Clausius-Mossotti relation??

what is the limitation for Clausius-Mossotti relation?? is that applicable for all the crystal structure??. If the material is an organic single crystal, how well this suits for it

31 January 2020 8,571 3 View

Does it require testing each topical formulation of our drug in laboratory animals, if drug has showed potential at some concentrations?

We have evaluated the wound healing potentials of a compound at a particular concentration in ointment form in laboratory animals. Now, we are preparing different types of topical formulations...

14 January 2020 2,893 6 View

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09 December 2019 8,735 11 View

Problem in downloading particular ZINC database file, suggestions?

Can anybody provide me with underneath Zinc database (.sdf format) https://zinc.docking.org/substances/subsets/biogenic/ or suggest me how to download this database. Although, I have tried many...

27 November 2019 7,112 8 View

Looking for a simple protocol of DNA Extraction from plant leaves? Suggestions please. Thanks in Advance?

Tried various protocols none is working. Generally getting DNA contaminated with phenols and proteins.

09 October 2019 8,548 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View