Selection of membrane is very important for expression of proteins with good quality in WB. So I want to know the best membrane and its supplier so that good results can be obtained.
PVDF membrane from Millipore is very good. My lab colleagues have been using it for a long time and it is working very well.
https://www.emdmillipore.com/US/en/product/Immobilon-P-Membrane%2C-PVDF%2C-0.45%C2%A0µm%2C-26.5%C2%A0cm-x%C2%A03.75%C2%A0m-roll,MM_NF-IPVH00010
Hello
You can Nitrocellulose membrane from Life technology
regards
PVDF membrane from Millipore is very good. My lab colleagues have been using it for a long time and it is working very well.
https://www.emdmillipore.com/US/en/product/Immobilon-P-Membrane%2C-PVDF%2C-0.45%C2%A0µm%2C-26.5%C2%A0cm-x%C2%A03.75%C2%A0m-roll,MM_NF-IPVH00010
I would also upvote for Millipore PVDF and we had terrible experience with GE healthcare membrane(would rate poor).
PVDF membranes from Biorad for big proteins. however if you are transfering a small protein (ie. 10 Kd>) then use nitrocellulose from biorad
Big proteins mean... My proteins of interest are within 12-100 Kd range.
There are actually quite a few variables that can affect the results of a western and influence the membrane of choice. As Fabrice noted, both can be utilized with excellent results. Your transfer efficiency for larger and smaller proteins will depend on the pore size, regardless of the membrane you use. Pall makes several different pore size for both NC and PVDF, and I've had success with both. Where PVDF does have a slight edge is when you have a large amount of background, as PVDF does a better job of eliminating background, in my opinion anyway. My rule of thumb, for new antibodies, I try both and see which works better, as I don't believe there's a hard and fast answer to your question, but I do agree with the brands suggested.
Thank you all for your valuable suggestions and sharing of your experience related to membranes for WB.
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