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Questions related from Sibasish Dutta
In this given paper (page no. 5, section 3.2, highlighted in green color) detection limit on dilution ratios are 3×10^5 and 12800 for AIV H7N9 and PCV2 antibodies respectively. Why are detection...
09 August 2020 9,679 2 View
What is the difference between Potentiometry, Voltammetry, Amperometry, chronoamperometry and Impedance Spectroscopy? Is there any relation between them?
29 June 2020 3,986 2 View
Darkfield and fluorescence microscopy
16 April 2020 9,275 1 View
Colloidal solution of gold and silver nanoparticles tend to agglomerate when centrifuged.
23 March 2017 6,958 4 View
In the coefficients of Mie scattering Riccati- Bessel function appears. What is the significance of these functions in the context of Mie -scattering?
16 March 2017 8,904 3 View
I have a white diffuser being illumined by a white LED (powered by 2.8 volt constant voltage) at the back of the diffuser. When I take images of the other side (front side) of the illumined...
25 February 2016 9,007 1 View
In doing colorimetric sensing using spectrophotometer, the replica (n=11) of a specific sample at a given concentration may produce large standard deviation in absorbance. How to control such errors?
25 February 2016 7,066 1 View
Any suggestions?
19 January 2016 5,252 2 View
Why different sized AuNPs (spherical) possesses varying degree of affinity for adsorption (binding) with BSA proteins or any other biomolecules?
11 January 2016 9,894 2 View
A collimated beam suffers from beam waist. When such a beam falls on grating the dispersion does not appear good. But this problem can be solved by the use of cylindrical lens how?
08 January 2016 8,330 10 View
Out of all the label-free techniques, How SPR and LSPR can be superior?
06 January 2016 3,408 4 View
I am attaching herewith the FTIR spectra of pure BSA, AuNPs (citrate capped) and BSA-AuNPs conjugates. It will be very helpful if somebody helps me in realizing the change in FTIR spectra for the...
04 January 2016 8,497 8 View
What are the changes takes place in FTIR spectrum of pure BSA and Trypsin enzyme upon binding to citrate capped gold nanoparticles individually? If anybody can give me some good references, it...
02 January 2016 1,637 3 View
What are the characterization technique available to ensure binding/ adsorption of BSA protein and Trypsin enzyme on citrate capped gold nanoparticles in colloidal form. It means I want to do the...
26 December 2015 4,333 14 View
I need to collimate light beam of an LED (white light) using a plano convex lens of focal length : 75 mm. If I place my LED at the focal plane (focal point) of the lens, will the lens collimate...
15 December 2015 7,066 14 View
Out of RGB, HSV, HSL,HSI, CIE 1931, CMYK and XYZ, which of these are color space and color models. Any help will be very useful.
09 December 2015 8,939 5 View
We know that for studying surface plasmon resonance (SPR), commercial SPR biosensor instruments are available. But for studying LSPR phenomena of metal NPs what are the other tools available apart...
03 December 2015 5,682 4 View
LSPR can be studied using noble metal nanoparticles in colloid form and through nanoparticle (of any shape and size) fabricated on a substrate through nanosphere lithography techniques. Can...
02 December 2015 6,715 3 View
Prism-based Kretschmann configuration SPR systems require polarizer for P-polarized light for exciting surface plasmons at the metal-dielectric surface while fiber-optics based SPR configuration...
01 December 2015 975 5 View
I have prepared 0.1 mg/mL BSA in distilled water. What can we convert 0.1 mg/mL into Molar unit?
29 November 2015 4,553 1 View
What is lowest amount of BSA protein that can be conjugated with citrate capped gold nanoparticles (20 nm) through direct binding (without using any linker)?I have prepared BSA stock solution of...
28 November 2015 8,005 5 View
I want to calculate x and y coordinate of a given color in CIE 1931 color space. What is the practical formula for that? I am attaching herewith the information I got from internet In order to...
26 November 2015 2,462 9 View
I want a uniformly illuminated surface over an area of 60 cm square area using a wide angle lens. What should be the specification for that wide angle lens?
17 November 2015 7,171 5 View
16 November 2015 6,553 1 View
I got the following preparation procedure (IN BOLD) in a paper for synthesis of gold nanoparticles of different sizes but I am confused with the term wt% of gold chloride (chlorauric acid) and...
20 October 2015 3,183 2 View
How can we extract the albumin as protein sample from egg white?
16 October 2015 8,608 8 View
I want to make colorimetric quantification of Lipase enzyme using Lowry's reagent. But while preparing the sample solutions (Lipase enzyme concentration of 1mg/ml) of different concentration of...
14 October 2015 9,410 7 View
I want to prepare stock solution of copper sulphate, sodium potassium tartarate and sodium carbonate and store it for future use. Is it possible to do so? If so, how should we preserve these stock...
13 October 2015 9,002 3 View
Upon fitting the data points I got an exponential fit to be best fitted curve. Can any one suggest me how to calculate the sensitivity in this case? I am attaching the graph.
18 August 2015 4,602 5 View
If a certain maxima appears in effective refractive index of a PCF; is there any possibility of getting a dip in confinement loss in PCF?
06 July 2015 7,864 1 View
If we use material other than silica in making Photonic crystal fiber, will it be possible to get a peak in effective refractive index in that material based PCF?
06 July 2015 3,728 2 View
I have 13 nm citrate capped gold nanoparticles colloids which I centrifuged at 17000 g. I would like to know that within how many hours should I redisperse it in a liquid media before the gold...
18 May 2015 5,966 3 View
Using a RAMAN spectrometer of incident wavelength 785 nm, can we determine the size increasing in spherical gold nanoparticles?
02 May 2015 9,006 4 View
For bioconjugation of citrate capped gold nanoparticles with BSA (prepared in PBS pH 7.4), I have added 200 micro liter of BSA solution to 1000 micro liter of AuNPs. But immediately after adding...
13 April 2015 4,641 7 View
Is it possible to observe redshift in plasmon resonance wavelength of gold nanoparticle colloids (13nm) by adding different concentration of glycerol to it?
07 April 2015 9,910 10 View
What is the effect of temparature on the absorption intensity of phenol red dye when treated with a buffer solution of specific pH?
25 March 2015 9,800 2 View
On mixing BSA protein powder in distilled water (0.01% - 0.04%) ant bubbles are being formed. How can I avoid this?
20 March 2015 8,257 8 View
In this research paper, a paper based microfluidic pad has been designed using n-paper(native paper). What is the meaning of n-paper (native paper). How is it different from ordinary paper? I am...
10 February 2015 443 2 View
I followed the following procedure for preparing gold seeds for growth of gold nanoparticles of different sizes as reported by Jana et al. I tried this procedure for 7 times but in all the cases I...
16 January 2015 1,906 8 View
How can we obtain a buffer solution of a given pH value by mixing two buffer solutions of different pH value?
04 December 2014 5,403 2 View
Chromic acid is very dangerous and spilling of this acid on floor can be very hazardous for the persons in the laboratory. Also pirhana solution and aqua regia requires large volume of acids which...
28 November 2014 7,040 5 View
Laser light is not fully monochromatic so is it possible to reduce its spectral width without using any monochromator?
20 November 2014 6,028 10 View
I am attaching herewith some very good papers for synthesis of monodisperse gold nanoparticles of different sizes. In almost all the papers same procedure is used for the preparation of gold nano...
17 November 2014 5,493 10 View
Binding of BSA or other proteins with gold and silver nanoaprticles causes shifts in LSPR wavelength.
07 November 2014 5,714 3 View
If I deposit silver/gold nanoparticles by EBL or some other techniques on a decladded optical fiber (U-shaped or straight) then when white light is allowed to pass through the optical fiber, will...
03 November 2014 5,906 7 View
When ever we take the absorption or transmission spectra of nobel metal nanoparticles using a UV-VIS spectrometer, there is a strong absorption due to the localsied surface palsmon resonance of...
17 October 2014 1,122 6 View
I tried to remove the stains of ferric chloride of my beaker with chromic acid but it didn't work out. Is there any way to completely clean glasswares stained with ferric chloride?
16 October 2014 5,633 18 View
I have tried to dissolve L-Cysteine in distilled water but I found it insoluble in distilled water. While googling I found that by mild heating and stirring or by dissolving L-cysteine in a water...
07 October 2014 8,710 11 View
There are a number of procedures available based on Turkevich method for synthesizing citrate capped silver nanoparticles but in every technique the size of the nanoaprticles generated are not...
16 September 2014 7,876 2 View
To obtain solid gold or silver nanoparticle, centrifugation of its colloids is required.
09 September 2014 4,957 27 View
The effective refractive index changes when metal nanoparticles are suspended in different dielectric mediums.
08 September 2014 3,059 4 View
To bind BSA (which is negatively charged) with gold and silver nanoparticles by electrostatic adsorption we need the surface of the nanoparticle( gold and silver) to be positively charged.
08 September 2014 2,377 8 View
To deposit silver or gold film of nanometer thickness, we need an expensive Vacuum coating machine. Since such expensive machines cannot be afforded everywhere, how can one deposit silver or gold...
06 September 2014 593 9 View
We know that when a white light passes through a transmission grating, the white light gets dispersed into spectrum.
04 September 2014 6,000 0 View
During preparation of gold nanoparticle colloids we need to weigh the gold chloride. During weighing of gold chloride it remains exposed to air for sometimes and during that procedure gold...
31 August 2014 253 6 View
It will be easy for the sample preparation of bio-conjugated gold and silver nanoparticles and show corresponding LSPR shift during teaching.
31 August 2014 2,415 6 View
Citrate capped gold nanoparticle colloids show an abrupt change in colour from ruby red to purple color upon dilution with PBS(Phosphate buffer saline).
29 August 2014 2,281 4 View
Physical adsorption of gold nanoparticle colloids with BSA protein.
15 April 2014 1,936 1 View
