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Questions related from Sibasish Dutta
In this given paper (page no. 5, section 3.2, highlighted in green color) detection limit on dilution ratios are 3×10^5 and 12800 for AIV H7N9 and PCV2 antibodies respectively. Why are detection...
09 August 2020 9,623 2 View
What is the difference between Potentiometry, Voltammetry, Amperometry, chronoamperometry and Impedance Spectroscopy? Is there any relation between them?
29 June 2020 3,923 2 View
Darkfield and fluorescence microscopy
16 April 2020 9,185 1 View
Colloidal solution of gold and silver nanoparticles tend to agglomerate when centrifuged.
23 March 2017 6,915 4 View
In the coefficients of Mie scattering Riccati- Bessel function appears. What is the significance of these functions in the context of Mie -scattering?
16 March 2017 8,834 3 View
I have a white diffuser being illumined by a white LED (powered by 2.8 volt constant voltage) at the back of the diffuser. When I take images of the other side (front side) of the illumined...
25 February 2016 8,967 1 View
Why different sized AuNPs (spherical) possesses varying degree of affinity for adsorption (binding) with BSA proteins or any other biomolecules?
11 January 2016 9,831 2 View
I am attaching herewith the FTIR spectra of pure BSA, AuNPs (citrate capped) and BSA-AuNPs conjugates. It will be very helpful if somebody helps me in realizing the change in FTIR spectra for the...
04 January 2016 8,440 8 View
I need to collimate light beam of an LED (white light) using a plano convex lens of focal length : 75 mm. If I place my LED at the focal plane (focal point) of the lens, will the lens collimate...
15 December 2015 7,017 14 View
We know that for studying surface plasmon resonance (SPR), commercial SPR biosensor instruments are available. But for studying LSPR phenomena of metal NPs what are the other tools available apart...
03 December 2015 5,639 4 View
LSPR can be studied using noble metal nanoparticles in colloid form and through nanoparticle (of any shape and size) fabricated on a substrate through nanosphere lithography techniques. Can...
02 December 2015 6,655 3 View
I have prepared 0.1 mg/mL BSA in distilled water. What can we convert 0.1 mg/mL into Molar unit?
29 November 2015 4,499 1 View
I want a uniformly illuminated surface over an area of 60 cm square area using a wide angle lens. What should be the specification for that wide angle lens?
17 November 2015 7,131 5 View
How can we extract the albumin as protein sample from egg white?
16 October 2015 8,557 8 View
I want to make colorimetric quantification of Lipase enzyme using Lowry's reagent. But while preparing the sample solutions (Lipase enzyme concentration of 1mg/ml) of different concentration of...
14 October 2015 9,333 7 View
I want to prepare stock solution of copper sulphate, sodium potassium tartarate and sodium carbonate and store it for future use. Is it possible to do so? If so, how should we preserve these stock...
13 October 2015 8,955 3 View
If a certain maxima appears in effective refractive index of a PCF; is there any possibility of getting a dip in confinement loss in PCF?
06 July 2015 7,816 1 View
If we use material other than silica in making Photonic crystal fiber, will it be possible to get a peak in effective refractive index in that material based PCF?
06 July 2015 3,679 2 View
What is the effect of temparature on the absorption intensity of phenol red dye when treated with a buffer solution of specific pH?
25 March 2015 9,744 2 View
How can we obtain a buffer solution of a given pH value by mixing two buffer solutions of different pH value?
04 December 2014 5,351 2 View
Laser light is not fully monochromatic so is it possible to reduce its spectral width without using any monochromator?
20 November 2014 5,968 10 View
Binding of BSA or other proteins with gold and silver nanoaprticles causes shifts in LSPR wavelength.
07 November 2014 5,664 3 View
I tried to remove the stains of ferric chloride of my beaker with chromic acid but it didn't work out. Is there any way to completely clean glasswares stained with ferric chloride?
16 October 2014 5,557 18 View
I have tried to dissolve L-Cysteine in distilled water but I found it insoluble in distilled water. While googling I found that by mild heating and stirring or by dissolving L-cysteine in a water...
07 October 2014 8,616 11 View
To obtain solid gold or silver nanoparticle, centrifugation of its colloids is required.
09 September 2014 4,841 27 View
The effective refractive index changes when metal nanoparticles are suspended in different dielectric mediums.
08 September 2014 2,991 4 View
To deposit silver or gold film of nanometer thickness, we need an expensive Vacuum coating machine. Since such expensive machines cannot be afforded everywhere, how can one deposit silver or gold...
06 September 2014 554 9 View
We know that when a white light passes through a transmission grating, the white light gets dispersed into spectrum.
04 September 2014 5,964 0 View
During preparation of gold nanoparticle colloids we need to weigh the gold chloride. During weighing of gold chloride it remains exposed to air for sometimes and during that procedure gold...
31 August 2014 202 6 View
Citrate capped gold nanoparticle colloids show an abrupt change in colour from ruby red to purple color upon dilution with PBS(Phosphate buffer saline).
29 August 2014 2,235 4 View
Physical adsorption of gold nanoparticle colloids with BSA protein.
15 April 2014 1,884 1 View
