Binding of BSA or other proteins with gold and silver nanoaprticles causes shifts in LSPR wavelength.
Please direct the addition of 1 uM BSA to a solution of citrate-capped gold nanoparticles.You will obtain BSA-modified gold nanoparticles. Use the same way for the modification of citrate-capped silver nanoparticles wth BSA
Thank you very much for your response. But can you please tell me what should be the volumetric ratio (in mL or micro liter) of citrate capped gold or silver nanoparticles to BSA?
Dear Sibasich.
Just use a concentration of mg/mL of BSA in water to have a full coverage of the citrate GNPs. You can speed up the reaction at pH 8 by adding 8 mL ethanol drop every minute when you stirred. An opalescent solution was observed indicating the formation of the nanoparticles. You need to add 8% glutaraldehyde solution for the stability of the particles. keep stirring overnight and then centrifuged at 15000 rpm for the nanoparticles to settle down. Remove the supernatant and you can lyophilize the pellets . After lyophilization, you can dissolved the particles in PBS buffer at pH 7.4 to prevent the denaturation of proteins.
Hope it will help .
Eric
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