79 Questions 166 Answers 0 Followers
Questions related from Kunal .
I have two year data and performed statistics on each year data individually. Now I got the mean and critical difference (CD) value of each year's data but I want to represent the data as mean...
02 February 2019 3,955 2 View
What do you think would happen if you added only 3 out of the 4 total types of dNTPs in PCR reaction?
10 October 2017 328 6 View
PCR or PCR plate has standard 96 or 384 wells (24 and 48 also available)? Why not it comes in different values. Regards.
08 August 2017 7,207 2 View
I want to give the RNase treatment in DNA extration. Can anybody please tell me the protocol for RNase buffer for the preparation of RNase solution? Thanks
09 September 2016 9,131 4 View
I have a problem of wavy running of DNA band in PAGE. Anyone please tell me why? Please check the attached photograph of the gel.
07 July 2016 4,755 6 View
Which online tool or software can be used as detection of Plagiarism in research papers or reports?
05 May 2016 7,294 5 View
Can anybody tell me what does it mean - Describe your research approach including how you ensure the quality of your work? What one can write in this? Please provide me some examples and...
04 April 2016 7,911 1 View
We are working on PAGE (Polyacrylamide Gel Electrophoresis) for resolving the amplified DNA samples of wheat crop. But when we tends to prepare PAGE gel, after its solidification, when we remove...
02 February 2016 1,566 11 View
For 16S rRNA sequencing to identify bacterial isolates there are several universal primers (forward and reverse) such as 8F, 1492R, 27F, 928F, 336R etc. Are these primers code for some gene? How...
07 July 2015 7,700 5 View
Is petrography for micro-structural analysis of mortar and concrete?
03 March 2015 799 8 View
Does the heat of hydration causes cracks in mortar and concrete? Please provide relevant literature. Thanks.
02 February 2015 1,620 15 View
What is the criteria for assigning the impact factor to journal? I have seen that some open access journals (not indexed in SCI) continuously flaunting their journal's impact factor without any...
02 February 2015 2,901 6 View
Alkalinity of cement can be determine by XRF or EDX in terms of % alkali content. How can we analyse quantitatively the alkalinity of concrete? Please suggest some protocol or literature. Thanks.
02 February 2015 6,446 3 View
It is reported that higher alkalinity is necessary for early hydration but higher alkalinity may for some products like ettringite, chloro- and sulfoaluminate phases etc. which weakens the cement...
02 February 2015 8,104 4 View
According to some literature there should be 0.6% alkalinity in the cement for use in concrete. As the high alkalinity enhanced the early hydration and strength, but affects the durability. Is...
02 February 2015 1,048 3 View
How can we determine the particular bacterial group or species form a mixed culture by using some chemicals? Is there any literature available on this? Please share your experiences and guide...
02 February 2015 2,275 7 View
Can you please suggest some water soluble organic calcium salts that can be autoclaved?
02 February 2015 6,502 8 View
Please suggest some methods to determine the hydration of cement without involving colorimetry? Thanks.
02 February 2015 3,424 4 View
Some literature reported that J-ring test for passing ability of self-compacting concrete is the difference between slump flow and J-ring flow diameter, which should normally be within 2 inches...
12 December 2014 4,080 2 View
While doing pesticide remediation of soil with bacterium, why it is difficult to remediate soil compared to water? Thanks for the suggestions.
11 November 2014 9,947 18 View
Please suggest me for testing facility centre for Thermo gravimetric analysis (TGA) in India? If anyone know about some centre in North India please tell me. Thanks,
10 October 2014 1,128 17 View
During writing a research paper/article we usually support our results with statistical analysis using t-test or ANOVA or other. How can we represent it in research paper? How to write discussion...
10 October 2014 9,880 3 View
Please, can anyone tell me what the role of TGA/DTA in the analysis of concrete is? What does it depict? Why do we TGA analysis on concrete? Thanks.
10 October 2014 1,873 6 View
Is it similar to bacterial growth curve? any procedure with reference for this? Thanks.
10 October 2014 4,933 7 View
Everyday new and novel researches takes place globally. I wonder how could one gets idea or think for such research? Does the change in few experimental parameters in already accomplished work is...
09 September 2014 9,372 7 View
What are the fundamental or basic things that one should keep in mind while writing a research or review paper? What are the parameters and strategies involved in it? Please share your suggestions...
09 September 2014 7,278 2 View
I need schematic representation or diagram regarding organic acid production by bacteria/fungi for bioremediation or metal leaching. Please provide me link or paper for that. Thanks
09 September 2014 543 1 View
I am confused about that is fermentation an aerobic or anaerobic process or both? I have read somewhere that "Fermentation does not necessarily have to be carried out in an anaerobic environment"...
09 September 2014 5,468 4 View
While doing biochemical identification or characterization of microbial isolate we uses carbohydrates as C-source in growth medium. What is the role of amino acids in growth medium? Are they acts...
09 September 2014 6,058 7 View
I want to know how much plagiarism (%) is acceptable for publishing a research paper or report or thesis? If I will use write up for my report from my already published paper then will it be come...
08 August 2014 6,706 9 View
XRD analysis of concrete containing rice husk ash.
07 July 2014 8,586 2 View
Please share your perspectives and experiences on the use of bacteria in concrete. Is it really an excellent tool for improving the properties of mortar and concrete? Is it cost effective? What...
07 July 2014 4,869 6 View
These days microbial concrete or self healing concrete is gaining much popularity for the bioremediation of cracks. Studies reported use of bacterial cells for such bioremediation by calcite...
07 July 2014 1,076 6 View
Measurement of pH of any solution is the physical or chemical process?
06 June 2014 4,510 9 View
I have read that hydrogen (generated by microorganisms) can be used as fuel for automobiles but its overproduction and rough handling may prove toxic and even lethal. What are the prospects in...
06 June 2014 7,050 4 View
How can we estimate the acetic and formic acid content in a solution using a UV-Vis spectrophotometer? If anyone has the protocol, kindly provide it as some protocols I have found on the internet...
06 June 2014 3,921 8 View
Is it possible to achieve this without affecting the strength and durability of structures? We know that raw and construction materials used in cement-concrete system are affecting our environment...
05 May 2014 8,544 8 View
This is just my opinion that PhD thesis should be the compilation of research papers with brief report instead of 100-200 pages report with detailed information. Generally the thesis contains all...
05 May 2014 4,630 1 View
Climate change showing the visible difference in the nature and ecosystem. Season variations (early/late summers or early/late winters) are the changes felt most. Does it affects the variations in...
05 May 2014 7,451 5 View
For last 5-6 years, the words bioenergy and biofuel are gaining much publicity, but we are still using non-renewable petroleum-based products as fuels. So much research has been done and currently...
05 May 2014 9,145 65 View
As both DNA and proteins are regarded as molecules.
05 May 2014 4,171 2 View
Some researchers have said that ettringite is very difficult to visualize on SEM because it decomposes early at short ages and if the concrete is 28 days old, there will be nearly no ettringite...
05 May 2014 8,984 3 View
What should one have to do reduce the environmental pollution? Certain things such as industries, automobiles are today's needs but they are the major cause of pollution... we can't close or stop...
05 May 2014 961 8 View
Generally HPLC and GCMS are used for determining the Polycyclic Aromatic Hydrocarbons (PAHs) in soil and water. Can we use a UV-Vis spectrophotometer for estimating PAH (either total PAH or...
05 May 2014 6,651 5 View
What is the best way to write a research paper so that the chances of rejection would be less? Any precautions? Please share your experiences. Thanks.
05 May 2014 823 15 View
Compressive strength of concrete is estimated by using UTM/CTM machine by breaking the specimen. Is there any technique by which the specimen remains intact and we can get the compressive strength?
04 April 2014 5,391 9 View
I have seen that tensile strength of concrete is always lower than compressive strength values (generally 10-12 folds). How can we enhance the tensile strength of the concrete? Is it possible to...
04 April 2014 7,147 13 View
It is generally saying that bacteria is immortal and never die. Is it true?
04 April 2014 3,181 6 View
Some journals (like those comes under Elsevier) have two types of policies. First is making your article subscribed (i.e. one have to pay fee for access), and second is paying the open access...
04 April 2014 7,309 15 View
I want to do some work on acidic soil and alkaline soil, but it is not available in my native place. How can we make neutral soil an acidic and/or alkaline one? Is there any chemical means or...
04 April 2014 8,948 8 View
Fly ash contains heavy metals. Studies reported the use of fly ash in agriculture but no study reveals how much fly ash can be used as a fertilizer in the next crop season. How can one estimate...
04 April 2014 907 3 View
Extremophilic microbes are those which can withstand harsh environments due to some modifications in their membranes, nucleic acids and proteins that provide them resistance against extreme...
04 April 2014 1,755 2 View
I am curious to know about the different perspectives of scientists on the use of biochemical characterization to identify microbes (bacteria/fungi/actinomycetes) when we have reliable molecular...
04 April 2014 8,349 21 View
In bioinformatics, we study about sequences and CpG islands where C&G base pairs are repeated many times. Why is there a need to study CpG islands in prokaryotes and eukaryotes? Is the study of...
04 April 2014 3,460 4 View
We do human and animal population surveys. According to http://www.worldometers.info/world-population/ as on April 06, 2014 world population is around 7.224 billions and animal population is...
04 April 2014 689 3 View
I have seen that there is a specific Rhizobium isolate for a specific nodule plant (such as Mesorhizobium for Chickpea; Bradyrhizobium for Soybean; etc.). What is the reason for such specificity?...
04 April 2014 9,899 5 View
Generally plants are considered as vegetarian diet whereas animals as non-vegetarian diet for humans. We inhale or intake bacteria and fungi (or fungal spores) in several ways like via food,...
04 April 2014 4,530 3 View
Is there any relationship between metals in cement with strength of concrete? Metals such as Cd, As, Ni, Zn, Cu affects the strength of mortar/concrete or not?
03 March 2014 1,696 2 View
What is the fate of heavy metals in concrete containing fly ash-Portland cement that was added (in which bacteria was added) during casting of the concrete? Is it similar to the process of...
03 March 2014 9,856 0 View
Is there any method or technique by which we can estimate the composition of cement mortar and concrete in terms of cement and sand content with unknown mix design? ** A case as an example** I...
03 March 2014 6,805 10 View
I have sequenced a 16S rDNA sequence and got one ab1 file of chromatogram. Please tell me which software or online tool I can use to check the chromatogram?
03 March 2014 7,245 16 View
If we insert an extra gene; similar to one already in bacteria having expression for particular pigment/antibiotic production/ nitrogen fixation/ PHB production etc.; in genomic DNA or in plasmid,...
03 March 2014 5,341 3 View
How can we check the purity of the gene sequence (in percent)? Is there any software or online tool or any calculation?
03 March 2014 6,931 13 View
When we take the average of two petriplates for counting the microbial colonies, sometimes the cfu count comes in fraction values, i.e. 0.5 x 10^8 or 2.5 x 10^8 cfu. But the fraction value is not...
03 March 2014 9,977 6 View
I want to keep myself update regarding the new technologies used or research to be conducted in the field of Microbial Diversity Studies (Mesophiles/Extremophiles) and Bioremediation Studies...
03 March 2014 4,104 2 View
We are working on isolation of oil degrading bacteria from different habitats but during preparation of growth medium when adding some concentration of oil it is not dissolved as it forms...
03 March 2014 1,677 15 View
We have isolated two bacterial isolates producing urease enzyme on Urea Broth/Agar growth medium with phenol red as indicator. During sub-culturing the colony from one broth to freahly prepared...
03 March 2014 1,977 5 View
I am keen to know about nano-bacteria. I have studied some papers on it and want to work in this field. Any one on ResearchGate have experience with nano-bacteria or is currently doing work in...
03 March 2014 7,902 1 View
I am worried that If we isolate thermophilic bacteria on agar medium, it needs to incubate at high temperature (>50 degree C). At such temperatures, the agar is going to melt or soften. How can we...
03 March 2014 4,263 9 View
I am looking for some simple literature for the procedures of EPA 846 - 1311, 1313 and 1315 methods for analysing the metal leachate behaviour in powder and solid samples. But the detailed methods...
03 March 2014 4,316 0 View
I want to work on some topic of industrial wastes (fly ash/sludges/incinerator ash etc.). As much work has been done and published on heavy metals in several wastes, what type of other work can...
03 March 2014 4,564 10 View
Would anyone clear my doubts about whether there is an alkaline or non-alkaline nature of cement/concrete causes corrosion in reinforced concrete? And how?
02 February 2014 6,516 11 View
If we already use less alkaline cement or use some non-alkaline cementitious materials in partial replacement of cement can it affect the corrosion (by increasing or decreasing it)? As decrease in...
02 February 2014 8,022 14 View
I have checked many research papers to find the %G+C content of whole bacterial genomes. Can the %G+C content of the whole bacterial genome be estimated by the %G+C content of the 16S rDNA...
02 February 2014 2,213 5 View
These days interdisciplinary research is getting more popular to search and re-search many new thing in different fields of science and engineering. I am working as PhD scholar and doing research...
02 February 2014 6,255 6 View
16S rDNA gene in bacteria is conserved in same genus of the family. I have identified a bacterium using 16S rDNA technique using universal forward and reverse primers. As genes also code for some...
02 February 2014 4,849 9 View
I have learned that for the identification of microbial isolate (especially bacterium) up to species/subspecies level one have to analyse 16S rRNA/rDNA sequence, %G+C content of the bacterial...
02 February 2014 9,631 3 View
Several times I found that most of the Non SCI and open access journals published papers on payment of fee. Whether they check the paper is of scientific relevant or not? They need only payment to...
02 February 2014 6,408 3 View
Sci-Hub is providing full text papers to scientific community without any fee. Is this a good thing or not? Please give you opinion about that.
01 January 1970 3,777 10 View
