I have a problem of wavy running of DNA band in PAGE. Anyone please tell me why? Please check the attached photograph of the gel.
Hi Kunal,
For me it seems that there was uneven distribution of the electric charge during the running. I don't know which type of chamber you were using for the run.
If the vertical one most likely there was a leakage from one side of your gel therefore the charge was uneven. You will commonly see this wave when you have this problem.
If it was horizontal one, you might want to check if your wire on the chamber still intact so that it produces even charge.
Hope it's help!
Yes, I agree with Sutandy. This is the common problem with old electrophoretic units, arises when electrode knobs are not tight. But, you should also prepare fresh buffer and especially check the pH of water used for buffer preparation.
another part, you can check your gel, if its even or there were problem with solidified agarose
hi,
i also face the same problem and the reason behind this was voltage fluctuation.
check the homogesity of gel (preparation) solidification check also the amonium persulfate if you use the PAGE as you said
also check the current , mAmpir and voltage the most imprtant the temprature during migration in relation with the concentration of acryl.
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