How can we determine the particular bacterial group or species form a mixed culture by using some chemicals? Is there any literature available on this?
Please share your experiences and guide me.
Thanks.
The identification of bavcterial species from mixed culture is long and complex and not to be undertaken lightly. Firstly, you must start by isolating the pure culture of the bacterial species you are interested in. This will require some good aseptic technique and suitable growth media. You should then try and identify the species using a sound microbiological / biochemical approach. The use of ealier editions of Bergey's Manual of Systematic Bacteriology and Cowan & Steele's manual may help with this. However, you should also be aware that many bacteria are said to be "viable but non culturable", hence you may also have to use molecular techniques to determine the presence of as yet unidentified species. 16s rRNA may also be useful if you have access to the technique.
It's called biochemical test, you can use biochemical test for differentiation of different groups of bacteria from eachother. for example, you can some staining dyes (such as gram, acid fast, ..) to determination of particular groups of bacteria. it's better to refer Microbiology laboratory manuals.
Ready test kits with selected biochemicals are available for characterization
Hello Kunal,
API galleries available from Biomerieux (www.biomerieux.com) are kits based on biochemical properties for identification of bacterial species. They were first developed for enterobacteria but galleries suitable for other bacterial genera are also available.
Good luck
The identification of bavcterial species from mixed culture is long and complex and not to be undertaken lightly. Firstly, you must start by isolating the pure culture of the bacterial species you are interested in. This will require some good aseptic technique and suitable growth media. You should then try and identify the species using a sound microbiological / biochemical approach. The use of ealier editions of Bergey's Manual of Systematic Bacteriology and Cowan & Steele's manual may help with this. However, you should also be aware that many bacteria are said to be "viable but non culturable", hence you may also have to use molecular techniques to determine the presence of as yet unidentified species. 16s rRNA may also be useful if you have access to the technique.
No direct chemical test is available which can give you the answer for particular group presence in mixed population. First requirement is to separate the each individual genera in form of isolated colony and then each individual genera can be identified by performing different biochemical tests. Now a days biochemical test kits are available and even one can use BIOLOG for further conformation of biochemical tests based identification.
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