I want to give the RNase treatment in DNA extration. Can anybody please tell me the protocol for RNase buffer for the preparation of RNase solution?
Thanks
Hi there,
A quick search on internet is giving the info:
http://cshprotocols.cshlp.org/content/2007/6/pdb.rec11030.full?text_only=true
Kunal, searching for a postdoc - I hope you learn to search well, soon!
RNase is one of the most stable enzymes. You can dissolve it in whatever suits your needs, including simply water for your stock.
One of things we routinely do to eliminate any contaminating enzyme activities, such as DNase, in the context of nucleus acid work, is to keep the solution of RNase in boiling water for ~30 min. Many enzymes, including DNase, are not so thermostable to survive 100C.
If you have searched well, I'm sure you have found all of the above and more. Now the question is when you say you need more clarity, what does clarity mean to you!
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