I have learned that for the identification of microbial isolate (especially bacterium) up to species/subspecies level one have to analyse 16S rRNA/rDNA sequence, %G+C content of the bacterial genome, DNA-DNA hybridisation and FAME analysis (as reported in many papers published in IJSEM journal).
What is the role of % G+C content in identification and what it means above 50% G+C and below 50% G+C content? which is better (above 50% or below 50% G+C) to relate the evolutionary relationship between isolated bacterial cell with other already reported . {>50% G+C content means evolutionary relationship or not?}
The G+C content is just a marker. Depending on the genus, a similar G+C content might or might not indicate a relation. For example, the next relatives of Corynebacterium glutamicum have a much higher G+C than this strain while more distant relatives have a similar one. So a certain G+C continent does not indicate relatedness.
To properly discriminate between species, you need methods with better resolution like the 16S sequence or DNA-DNA hybridization.
The G+C content is just a marker. Depending on the genus, a similar G+C content might or might not indicate a relation. For example, the next relatives of Corynebacterium glutamicum have a much higher G+C than this strain while more distant relatives have a similar one. So a certain G+C continent does not indicate relatedness.
To properly discriminate between species, you need methods with better resolution like the 16S sequence or DNA-DNA hybridization.
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