Is it similar to bacterial growth curve? any procedure with reference for this?
Thanks.
Doubling or generation time is calculated from log phase of batch microbial growth. Please find the link below:
http://www.doubling-time.com/compute.php
Doubling time is Generation time
Generation times for some common bacteria under optimal conditions of growth.
Bacterium Medium Improvement Generation Time (minutes) 0ptimum yield
or Chemically Defined Medium
Acetobacter Xylinum Add Pineapple juice 480-600 static
pH4 0.5% Amm Sulfate 240-360Agitated and aerated
120- shakers
Aspergillus niger 450
Bacillus licheniformis 300
Bacillus Polymixa NH4 180
Bacillus subtilis 75-120
Bacillus megaterium Sucrose-salts 25
Escherichia coli Glucose-salts 17
L Lactis pH 4.5 145
Lactic Acid Bacillus pH 7.5 180
Lactobacillus acidophilus Milk, Specific medium 66-87
Lactobacillus bulgaricus New CDM 60
Mycobacterium tuberculosis Synthetic 792-932
Nitrobacter 600
Nitrosomonas 480
Pseudomonas flourescens 47
Rhizobium japonicum Mannitol-salts-yeast extract 344-461
Staphylococcus aureus Heart infusion broth 27-30
Streptococcus lactis Milk 26
Streptococcus lactis Lactose broth 48
Treponema pallidum Rabbit testes 1980
Sacharomyces cerevisiae New CDM 360
Thiobacillus ferroxidans pH 3-5.8 300
Acidithiobacillus ferrooxidans pH 2-3.5 H2S 250
Thiobacilus thiooxidans 500mg Tween 80 250
you can use optical density or direct plating method to evaluate bacteria amount in liquid medium. When you get the growth curve, you can pick up starting and final points during the exponential growth, and find average time for 2-folds difference of bacteria number. But it will be true for this experiment only.
Look at: http://faculty.sdmiramar.edu/dtrubovitz/micro/microhandouts/labs/growthcurve.pdf
What sort of analysis are you thinking of - and why?
Bacterial growth is affected by anything - temperature, nutrient levels, pH, age of population, genus, species, variety etc., etc. Any population of bacteria will be heterogeneous and you can determine only the mean generation time unless you watch a single cell under a microscope but many bacteria produce extracellular growth regulators (antibiotics) that probably affect their own growth rate. The times taken for a population of bacillus spores to germinate follows a Weibull distribution in which YT, the fraction gemnated in time T, is given by YT = exp(-BT-R). R defines the shape and B the scale. Experimental results suggest that R=2, which gives an asymmetric shape and a Rayleigh distribution.
I suspect hat most functions of most bacteria have a similar dsitribution.
Doubling or generation time is calculated from log phase of batch microbial growth. Please find the link below:
http://www.doubling-time.com/compute.php
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