Can you suggest me why we remove stop codon when protein sequence is to be analysed and classified in Interpro?

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Treatment of a large cross sectional unit in a panel setting?

I have a data set of 5 countries for 30 years and i want to analyse the relationship between y and x in panel setting. However, the countries are not similar in nature moreover. one country is...

30 January 2021 6,564 4 View

What is the solubility of Magnesium trifluoromethanesulfonate in methanol?

I am trying to dissolve Magnesium triflate in methanol. I took 2.6 g of it in about 20 mL methanol. After thorough stirring, I can see suspended particles! Can anybody throw some light on its...

12 January 2021 6,481 2 View

Could you explain the following situations, please?

I am trying to simulate a flexible antenna on ANSYS HFSS. Here, I have used "PDMS" as a substrate and "pec" as a probe (inner conductor) of a coaxial feeding. But during simulation, it is showing...

24 November 2020 800 5 View

Can I get an explanation for the following reason, please?

My plan is to fabricate a conformal microstrip patch antenna. I am going through the simulation works first. I already simulated the planar antenna. Now I am trying to simulate it with different...

19 November 2020 2,961 2 View

What are the community-based primary prevention of NCD in LMIC country?

Please suggest types of literature to look for? Recommend journals which focuses on these issues.

12 October 2020 2,878 4 View

How write a concept note on Evaluation of community-based primary prevention models for NCDs?

1. What type of literature should I read for writing a successful concept note? 2. Is there any literature which will help me decide a research question/objective for designing a study will be...

12 October 2020 3,363 5 View

Which software might be helpful to design and simulate the 3D shape of the planar/nonplanar microstrip/any antenna?

I am experienced with the antenna simulation using ANSYS HFSS and CST. But now I am working in an additive microsystems lab and want to move to 3D shape antenna simulation and printing.

03 August 2020 2,184 3 View

If I want to print the microstrip antenna using additive manufacturing techniques, do I need to design a 3D shape on CAD that I have created on HFSS?

I have designed and simulated a frequency reconfigurable on ANSYS HFSS, and now I want to print it using an inkjet-printing technology.

03 August 2020 7,439 4 View

What will be the best methodology to quantify Demographic Dividend and Skill labour of any country?

Most of the researchers used panel data to show the impacts of demographic dividend. But for individual country where divisional or district data over the year are not available, what methodology...

29 July 2020 6,733 1 View

Discrete Element Method Simulation Example Using Ls-Dyna?

I am interested to simulate the fracture process of glass using DEM in Ls-Dyna and need some example or bench mark case for learning.

21 July 2020 4,156 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View