I want to design a single primer pair for a particular genus, involving its 5 species, so that while performing PCR, we involve a single primer pair, instead of 6 primer pairs.
Ariel Vina-Rodriguez
Of course... first be sure it has not been published before.
If you dont find any better software that can help you, you may try my own solutions:
- from already aligned sequences:
https://github.com/qPCR4vir/VisualOliDeg
- whithout alignment, and directly from a bulk BLAST search:
https://github.com/qPCR4vir/ThDySec
This help you pick candidate primers and probes, that you will need to check with a known software, like primer3.
Let me known if you have problems with that, I will try to help.
2 votes
0 thanks
- Badges
- Science method
- Similar topics
- Biological Science
- Genetics
- Genetic Analysis
- genetic techniques
- Sequencing
More Sonalika Kar's questions See All
Similar questions and discussions