09 April 2017 1 9K Report

....?

....?

More Daya Materazzie's questions See All
Why pGEM vector do not digest?Why it is better than pUC 19 vector?

briefly explain

26 July 2023 408 0 View

Expression vectors are often used in Bacillus subtilis rather than E. coli. Why?

briefly explain

15 April 2023 7,766 2 View

Are mutations on introns do not affect gene expression?

want a brief explain for this

24 March 2023 2,053 4 View

Can someone convert this DIgSILENT PowerFactory example to an older version?

The attached example from the DIgSILENT website has been created with version 16 which I can't access. I would appreciate it very much if someone with the PowerFactory import the attached example...

14 April 2020 7,822 2 View

How to write arduino coding for frequency counter circuit?

I've been trying to write Arduino coding for this circuit but it still not successful. I hope anyone can help me. This circuit basically is about to detect the unknown capacitance of oil that...

06 April 2020 10,088 3 View

Is there any change in RevMan Review Manager 5.3 version especially use of +, - and ? symbols for low, high and unclear risk of bias?

I need some help to adjust +, -, and ? symbols for low, high and unclear risk of bias for Cochrane risk of bias assessment in meta-analysis using RevMan Review Manager software 5.3. When I...

22 March 2018 6,563 2 View

Is any experimental data avalible for SCWR forced flow system?

I am working on the instability analysis of the Supercritical Water cooled Reactor(SCWR). My work is computational using MATLAB. I have developed the mathematical model for thermal hydraulic+fuel...

31 May 2015 6,170 5 View

How can I get poincare section and lyapunov exponent using MATLAB?

I would like to compute poincare section and lyapunov exponent for my mathematical dynamic model. I am using MATLAB for the analysis, it would be very helpful for me if anyone provide any code,...

17 May 2015 9,441 3 View

How to do Bifucartaion analysis in MATLAB?

I want to do Bifurcation analysis for reactor dynamics, If any one using MATLAB for this  analysis then please help or suggestion some paper or text. Thanks in advance

28 January 2015 2,354 8 View

Why we study instability generally in natural circulation instead of force circulation reactor?

As there is very less instability in forced circulation reactor rather than natural circulation system.

06 January 2015 9,987 7 View

Similar questions and discussions
Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close