we have performed assembly using both CLC work bench (average length: 674 bp; N50: 890 bp) and TRINITY (average length: 725 bp; N50: 928 bp) and performed the analysis from both of them subsequently. On performing the annotation of both the assembly file and comparing the both, some annotated transcripts are showing more alignment length in TRINITY while some are CLC assembled sequences however differential expression from both of them showed similar type of pattern. So is it feasible or acceptable by the manuscript if we use both of the assembly files and consider the transcripts/Contigs with highest alignment length in the study.
I think it would be a good idea if you can merge your assemblies. For example:
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0091776
Hi,
I think its acceptable if you make sense of why you used this rather than that.
I think it would be a good idea if you can merge your assemblies. For example:
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0091776
- Badges
- Science method
- Similar topics
- Biological Science
- Genetics
- Genetic Analysis
- genetic techniques
- Sequencing