I mean sequencing + assembling. The genome of that species has been already sequenced.
It depends which technology to use (NGS, Pac-Bio etc).
If you consider raw data coverage, for same amount of coverage NGS would be cheaper but harder to work with than Pac-Bio. (assembly contingency)
With pac bio,I believe around 40x-50x coverage for an average bacteria genome you would easily get chromosome level assembly using CANU.
(http://canu.readthedocs.io/en/latest/quick-start.html#assembling-pacbio-data)
The price of sequencing varies. If I were you I would connect directly with the companies.
Dear Bogdan,
I recommend you Macrogen. They offer sequencing on many platforms.
I would choose SMRT (Pac-Bio etc), if you have only one candidate for sequencing.
Hi, I guess it depends on which platform and company you choose. For example, Sanger is cheap but others such as Myseq is more expensive but more reliable method.
Hi, I have in the lab a TSQ Quantumm access Max triplequad mass spec. since last year it was not opened. Had a problem regarding the ion gauge. exchanged it. more, now, I have a problem with...
07 May 2024 6,109 0 View
I'm trying to synthesise a product through suzuki coupling using 5-bromo-2-thiophenecarboxylic acid and 2-fluoro-4-methoxyphenylboronic acid. I tried using Cs2CO3, K2CO3, Na2CO3 as base and...
21 February 2024 4,725 1 View
I tried to obtain TiO2 nanoparticles by combining a solution of titanium isopropoxide (TTIP 1 mM, dissolved in isopropyl alcohol) and epigallocatechin gallate (EGCG 1 mM, in water), but, even...
17 January 2023 9,374 2 View
I have come across several published papers that report electrochemical experiments performed in electrolyte solutions in which the supporting electrolyte is 0.1M PBS (pH = 7) + 0.1M KCl. However,...
08 December 2021 4,894 0 View
Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...
03 February 2021 3,470 1 View
In last years, web development added new layers of opportunities for research, as more metrics like loading time, payload and resources requests are defined. In that case, the development has new...
01 November 2020 8,275 7 View
What is the best approach to identify TFs in a non-model organism with limited homology to the closest model organism? Is predicting DNA-binding domains and assuming that most proteins containing...
09 July 2020 5,462 2 View
Quick question, I am really stuck... So, my measurement model in AMOS has great goodness-of fit indicators, and the coefficients are highly significant. However, the error variances are immense....
20 April 2020 8,042 6 View
Наша команда дослідників працює над визначенням рівня втоми спортсмена в різних режимах роботи та над методиками їх відновлення. Мене цікавить відстеження руху зіниці ока в різних станах (стан...
04 September 2019 510 2 View
I am interested in a software that generates the network events data sent by a mobile phone to the surroundings antennae. At least a good tutorial about what data the mobile phone sends to the...
15 March 2019 5,090 5 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View