I want to amplify mer genes from bacteria. Can you suggest any primer or software for primer designing?
Hello Binish,
I suggest you Primer3 or Oligo7 Primer Analysis Softwares :)
Primer3 is the golden standard, and free to boot. Beware it's a command-line application, but there are graphical interfaces around the web.
Oligo is excellent, but this is commercial (and rather expensive) software.
Dear
These could be help
https://www.ncbi.nlm.nih.gov/tools/primer-blast/
http://simgene.com/Primer3
http://biocompute.bmi.ac.cn/CZlab/MFEprimer-2.0/
http://www2.uni-jena.de/biologie/mikrobio/tipps/rapd.html
http://www.premierbiosoft.com/tech_notes/PCR_Primer_Design.html
this maybe help you
http://pyromark-assay-design.software.informer.com/2.0/
this may be helpful
Gene runner is also an useful softaware for primer designing...after designing a primer we can also check its property that will fit for PCR reaction using the website http://www.bioinformatics.org/sms2/pcr_primer_stats.html ...in this website in the box delete what that are written & just copy your primer sequence into it & click submit...it tells you about the properties that your primer passes...
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