We have sequenced Synechococcus sp. PCC 7002 genome. Already the same strain has been sequenced and annotated by many before. Whole-genome map is also available online. The gene of interest is not present in NCBI gene bank assembly of the same strain. What we want to know is that wether our strain has been mutated and whether it has this particular gene or not? I have assembled and annotated the sequenced files using velvet and Prokka but do not know the further exact procedure to get efficient results.

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