What are the current best options to deplete rRNA in Arabidopsis?

Alexandre Vicente @Alexandre-Vicente-4

02 June 2020 1 1K Report

I am interested in mRNAs only (also the ones from chloroplast).

RNA input x RNA yielded approximately?

Additional Kit used to concentrate RNAs + to get rid of other RNAs (tRNAs, etc...)?

Thank you in advance.

Alexandre Magno Vicente

Katarzyna Knop

Hi Alexander,

you can try to perform either double mRNA pull down (after the first round of mRNA pull down just do another pull down with new beads' batch - for me Dynabeads from Thermofisher work really well) or you can try ribo-depletion using rRNA depletion/removal kit (e.g. NebNEXT one). I hope that will help you.

Kasia

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