I am new to HMMs and was thinking to build new HMMs based on the NCBI database(which is available to download) or SILVA, so that it can be used by some other program. Can you suggest any tool which can easily serve my purpose?
Use HMMER and its command hmmbuild to make profile hmm
02 March 2021 7,082 3 View
I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...
01 March 2021 1,587 6 View
I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...
27 February 2021 4,703 1 View
Greetings to all of You! I have searched some research papers for the designing of a proximity coupled patch antenna. Researchers are talking about the impedance bandwidth of the antenna but...
24 February 2021 7,662 3 View
This insect is minute in size around 1 mm and not clearly visible with naked eye.
22 February 2021 5,907 11 View
I am working on a biosensing project on paper substrate, however, it is found that the RNase contamination occurs on the filter paper. Might I ask is there any method to clean the paper surface...
12 February 2021 3,926 3 View
Hi everyone, I would really appreciate it if someone can tell me when and why a raw fluorescence starts below zero. In the picture attached below the blue colour curve starts from a negative...
09 February 2021 4,642 3 View
I have synthesized LiMgF3 using the starting precursors LiF and MgF2 via solid state diffusion method. Now I am looking for some characterization to claim the desired product, as there is no...
09 February 2021 921 3 View
This is a follow-up on an earlier question regarding mechanistics of vaccines: why current vaccines don't block transmission. In summary, they don't engage mucosal immune cells to produce IgA....
08 February 2021 8,321 3 View
Hi everyone, I am looking for fluorescence expression vectors for expression in gram-positive bacteria present in the skin microbiome. I need to tag them with a fluorescence marker and observe...
06 February 2021 2,566 4 View
Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?
01 March 2021 3,422 3 View
I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?
21 February 2021 7,783 12 View
I'm currently working on the paper The Green Solow model of William A. Brock and M. Scott Taylor. In order to create my models, I'm currently seeking for a database where one can find the...
24 January 2021 5,342 4 View
I have annotated a genome successfully with PROKKA. I can upload the genome to NCBI, but I cannot upload the annotation. PROKKA does not produce files in the correct format, and I cannot find any...
29 December 2020 3,976 3 View
How to use NCBI to design primers for specific Micro RNAs for quantitative analysis and how to check the efficacy of the selected primer before working
24 December 2020 180 3 View
Right now, I want to design qPCR primer for my target gene. But that gene has 21 predicted mRNA sequence in the NCBI database. Most of the sequence names are same but only different location...
23 December 2020 5,531 12 View
I want to know all prescription drugs which are FDA approved compounds for infection like Urinary Tract Infections along with their causative agents. Can you guide me about any resource, database...
17 December 2020 4,852 3 View
Hi I have a database in which there are several dates indexed as strings and in Shamsi format. I need them as dates (not strings) and prefer them to be Christian. I have tried "Date and Time...
14 December 2020 2,798 3 View
I have sequenced the mitochondrial genome of some moths species. I want to annotate and the sequence will submit in the NCBI.
19 November 2020 8,849 1 View
I'm asking and posing this question as it forms a part of my final project year. I building an ASP.net community-based website coupled with an Oracle database and I'm looking for feedback,...
17 November 2020 7,852 1 View