Does anybody have any literature that explains how to determine how many sequences I need per sample?

23 January 2020 1 930 Report

I am trying to figure out how many sequences I need for my samples. So far I can see that 40,000 would be necessary for microbial soil analysis. I have iDNA samples and so think I will need ~20,000 sequences however I cannot find any literature to support this.

Frederic Lepretre

Hi Buffy

it depends mainly on some parameters as:

- the size of the sequence you target

- the question you want to fix (higher coverage for rare events to be detected)

- the power of the machine you'll sequence on

- the number of samples you'll multiplex

the formula can be set as:

number of requested sequence = Nb(samples) * size(targets) * coverage

fred

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