How can eggs and larva extracted from fecal sample which is preserved in potassium dichromate and those eggs and larva are needed for DNA extraction?

14 June 2025 1 2K Report

Fecal samples collected from wild animals. preserved in potassium dichromate and stored at 4 degrees centigrade. DNA extraction is needed for host identification and helminth identification.

Abdelhak Maghchiche

To extract eggs and larvae from potassium dichromate-preserved fecal samples, centrifuge and wash thoroughly to remove the preservative, then lyse the eggs/larvae using enzymatic and mechanical methods before DNA extraction. Removing potassium dichromate is essential to prevent PCR inhibition for successful host and helminth identification.

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