How to increase the expression level of a yeast surface display peptide library (percentage of cells displaying the nanobody on its surface )?

Aritra Chattopadhyaya @Aritra-Chattopadhyaya

09 June 2025 0 1K Report

I AM SCREENING A NANOBODY LIBRARY AND THE EXPRESSION IS UNDER GAL PROMOTER , THE INDUCTION IS DONE UNDER GAL PROMOTER AT 25 DEGREE C THE REPORTED EXPRESSION LEVEL IS 15% OF THE WHOLE POPULATION BUT MY LEVELS ARE ONLY 10 % , the plasmid is pyd backbone

the expression level is checked using immunostaining against ha tag

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Science method

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