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Questions related to Scanning Electron Microscopy
We have crystallized a native protein; it lacks significant homology to any other known structures. We have been trying to crystallize the SeMet-substituted protein, but have difficulties to...
16 June 2020 6,778 1 View
Dear forum, I am currently working on a project where I have two groups (A and B), and I want to compare if a relationship between an IV and a DV significantly differs across the two...
12 June 2020 3,530 3 View
It can’t be an SD or SEM because i only plotted the test sample concentration and the computed enzyme activity. Thank you.
06 June 2020 9,230 1 View
We have ran a project looking to reduce number of blood tests on wards. (data is about a thousand patients each time) Looking at all the patients on medical wards over 1 month, measuring number...
05 June 2020 4,705 0 View
For SEM-based analysis of trichomes (soybean plant) can we preserve the samples collected for some period of time? or after following standard fixation procedures it can be preserved under low...
02 June 2020 5,916 1 View
Dear Researchers: Greetings! I am interested in learning electron microscopy image analysis of bacterial biofilms, mainly using open-source software. I have been using basic functions and tool...
29 May 2020 5,824 4 View
Hello, I have questions about XL30 SEM (with tungsten filament). Can anybody help me with how to correct crossover? You will see in the picture look of my crossover. I trying everything and can't...
25 May 2020 1,683 3 View
I am an undergraduate. My research is to find solution for control algal bloom
22 May 2020 6,631 3 View
I am trying to simulate interaction of hemoglobin with silver and gold nano particles using NAMD. However, I couldn't find any suitable potential. Does anyone have any idea to simulate the...
08 May 2020 8,024 0 View
I have tried PAA and F-127 to disperse TiO2 nanowires after aggressive probe-sonication which resulted in smaller aggregates upto few microns instead of individually dispersed as seen from DLS,...
05 May 2020 7,735 3 View
Dear EBSD friends, I am wondering what the possibilities are for EBSD without sample tilt. I vaguely remember a paper (or conference talk) where this was done. I think they used a detector which...
02 April 2020 932 10 View
Dear all, I am running an SEM in R. However, the model does not fit with reporting 'lavaan WARNING: some estimated lv variances are negative'. Any suggestion or solution? I guess the problem...
30 March 2020 9,741 4 View
I conducted an experiment of depositing zinc on a glass substrate using dc magnetron sputtering. and I found through SEM that zinc was deposited as a porous structure under pressures of 100-300...
20 March 2020 5,190 3 View
I have prepared water in oil in water double nanoemulsions samples .The formulation consist of sample extract 25mg + 4% oil + 2% Tween20 + 1% Chitosan(90% deacetylated). DLS results show Mean...
04 March 2020 3,906 4 View
Using standard commands, I manage to plot an orientation map just along the ND direction.
26 February 2020 9,147 3 View
If I want to do a SEM (Scanning Electron Microscopic) study on Dental implant surface, do i need to cut the implant to make a block, a thin strip or the Whole implant can be sent to lab for SEM?...
25 February 2020 8,039 7 View
Hi, Can anyone with experience on working with Rapidly solidified melt spun alloys kindly let me know how to prepare SEM samples out of the same, as they ribbons are too thin (around 30 microns)...
05 February 2020 4,492 6 View
For a conventional tungsten-filament "electron gun" used in electron microscopes they say the Wehnelt cap suppress electron emission from the filament at the sides and base, concentrating emission...
27 January 2020 8,675 4 View
TEM is not available. Also, should sample be centrifuged or not and what dilutions are required ? Kindly guide me on this.
26 January 2020 1,365 9 View
Which steps should I follow to dehydration and drying before SEM evaluation. The device i’ve use for this study is a new-generation product of Hitachi btw.
23 January 2020 1,915 8 View
1. Can JurkaT cells be used to replace primary human T lymphocytes for the study of T cell dysfunction? 2. How to induce JurkaT cells dysfunction or exhausted? 3. Can JurkaT cells differentiate...
10 January 2020 1,215 1 View
I have used DWLS method in CFA/SEM because my data is ordinal (7-Likert scale) and I have therefore estimated the polychoric correlations. Hu and Bentler's (1999) fit cut-offs were developed for...
03 January 2020 5,444 9 View
Dear all, I have coated MnCO3 microparticles with different biopolymers through Layer-by-layer approach. I am not sure how to analyze the sample under SEM. I fear sputtering might damage the...
03 December 2019 2,339 4 View
I obtained protein crystals using the MIDAS Kit under the following condition: 30% v/v glycerol ethoxylate, 0.1 M Mes pH 6.5 and 0.2 M ammonium acetate. I would like to know if this condition is...
22 November 2019 6,619 3 View