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Questions related to Scanning Electron Microscopy
Dear Researchers, "I am currently culturing Caco-2 cells in Transwell inserts using a 12-insert system with a 24-well plate (0.4 µm pore size, PET membrane, 0.33 cm² growth area, NES brand). I...
5 weeks ago 5,520 3 View
Can you please let me know who is using these cell lines and able to provide the cell line for my study. OVCAR8; CAOV3; OVCAR3
07 August 2025 9,227 1 View
Suggest a better software for pegrna and sgrna design. I'm trying to use docker and so on as per how it's mentioned in GitHub to use Primedesign by pinellolab. I could not get CFD score in this....
30 May 2025 3,173 0 View
I read a paper using lipo2000 to co-transfect cas9 mRNA and sgRNA, but I failed when using lipo3000. I followed Thermo fisher's protocol (without using P3000), the cell line was HepG2.2.15. Do I...
20 May 2025 9,019 0 View
I ran a 4–12% SDS-PAGE gel containing four 1:2 dilutions of myosin-actin standard and BSA combined, along with four additional dilutions of a human diaphragm digest. It’s unclear to me which...
31 March 2025 2,780 0 View
We want to fraction the protein components in our sample based on the molecular weight .But unfortunately the total protein concentration is low (20microgram/mL),so it is difficult to use gel...
16 March 2025 6,472 0 View
How to develop targeted treatment plans given the differences in responses to medical aesthetic treatments among people of different ages and skin types?
16 December 2024 6,257 0 View
Explain and discuss here....
18 October 2024 3,577 2 View
When I thawed PBMC, after 1 day I observed aggregation of cells. Viability was 90% when I thawed
23 July 2024 1,207 4 View
My liposome is a non-phospholipid liposome (sterosome) composed of palmitic acid and cholesterol, 20mg of palmitic acid and 70mg of cholesterol. After several months of storage in a refrigerator...
19 June 2024 7,528 5 View
I'm using TOPAS for quantitative analysis by Rietveld refinement. In many cases, amorphous is present in my samples. What will be the consequences if the density of the amorphous phase differs...
18 June 2024 2,051 4 View
Whenever I mill silica gel with other materials in stainless steel milling jar, the color of the powder goes from white (initial) to dark and darker color like yellow, dark yellow, brown, dark...
17 June 2024 4,074 3 View
Hi, I am doing analysis of Transystem inc. Downconverter model AiDC+1746D with RF=2216-2300MHz to IF=470-554MHz. The issue that I am unable to find frequency synthesizer and mixer, only 6.8MHz...
10 June 2024 8,455 7 View
Hi everyone, I really don't know how to fix the issue that I am facing. For background, I am working on the purification of inclusion bodies protein, which require the use of 8 M urea in the...
10 June 2024 9,611 0 View
I prepared a maghemite sample. It is perfectly reacted to a magnet. However, when I tried to analyze with XRD, I did not get any meaningful peak. What is the reason for that?
10 June 2024 3,311 3 View
Hi everyone, I have a question that has been troubling the whole lab here with the SDS-PAGE gel staining. We usually use colloidal gel staining (ROTI blue quick) and it works fine every time....
23 April 2024 5,736 0 View
Hi everyone, Does anyone happen to know the solubility of casein in urea solution? I have a problem with the purification of recombinant casein as most of the protein are produced in inclusion...
22 February 2024 4,935 3 View
I am facing difficulty on removing text like XYZ.PRF:, when we run fullprof in External applications in winplotr. Is there some way to remove it?
06 December 2023 6,655 6 View
I was thinking about Teensy 3.5, because it has DAC, but it is discontinued, unfortunately. Raspberry Pi Pico seems interesting, but it has PWMs only, which can be converted to DC voltage signal...
03 November 2023 2,242 4 View
We are trying to measure degree of conversion of resin composite under ceramic restoration using FTIR. The specimens are flat and are cured next to a cellophane strip, so they are smooth and...
01 November 2023 4,007 4 View
the equations are from "Nanosecond mid-infrared pulse generation via modulated thermal emissivity" but I cannot use mathmatica to solve it .
22 October 2023 3,135 0 View
using the telegraph's equation
18 October 2023 7,117 0 View
I want to analyse my samples in Vibrating Sample Magnetometer. M vs T and M vs H. I couldn't find VSM working anywhere in Tamilnadu right now. If anyone knows about it, please reply. If not in...
08 October 2023 952 1 View
and how many kpoints should we select for a 2x2x2 supercell having 52 atoms for phonopy calculations?
18 September 2023 476 2 View