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Questions related to Scanning Electron Microscopy
We require a method that will keep the sample in tact while removing it from Wax embedding, so that it can then be embedded in EPON in order to avoid damage to the instrument during scanning...
20 November 2019 10,049 2 View
Hi! According to our knowledge, osteopontin (opn) is an extracellular protein. Yet when we discussed the staining image today, some colleagues insisted that opn is intracellullar, surrounding the...
19 November 2019 776 3 View
We used to use SEM to characterize the samples prepared by us. In order to do this, we have to send our samples to somebody, get characterized, and let them send back. This takes weeks. To...
15 November 2019 3,758 3 View
Hello, I'm working with pulsed electric field applied to lemongrass leaves to generate pores on the cell membrane to enhance essential oil extraction. I would like to know if there is a way to...
21 October 2019 1,560 3 View
Hello! I have Al 6082 samples (strongly deformed by extrusion and partially recrystallized) and I would like to do an EBSD analysis of the recrystallized zone and have already tested the...
19 October 2019 6,691 4 View
I operate JEOL JSM 7001F. I always found it hard to get good quality images while studying glass substrate (and other non-conductable substrates) samples due to charge accumulation. Now there's a...
15 October 2019 4,183 4 View
These are areas of different samples of corroded mild steel, one is given a corrosion inhibitor at 40 C and one is given the same inhibitor at 90 C. The EDS results aren't that much different, but...
06 October 2019 8,388 13 View
I am planning inplanta bioassays by using endophytic BCAs against bacterial pathogens to see its efficacy and mode of action. Previous work illustrated good results invitro and inplanta by SEM...
02 October 2019 7,638 3 View
Hi everybody, I want to know, does in-vitro degradation of PCL causes an acidic environment? Thanks in advance.
01 October 2019 9,852 1 View
Hi, and how to preapre a sample with and without fouling for the imaging ?
17 September 2019 3,787 2 View
I'm working on alginate-based hydrogels and I want to observe the microstructures (porosity, roughness, etc.). The hydrogels were produced by gelation in aqueous calcium chloride. SEM is out of...
31 August 2019 3,080 4 View
I want to take the SEM and TEM images of samples I synthesized. I synthesized g-C3N4 (Graphitic Carbon Nitride). If I need to take SEM and TEM, should I disperse the obtained powder in a medium...
24 August 2019 2,175 9 View
I would like to analysis the S. aureus biofilms to know the effect of certain quorum sensing inhibitor on ultra structure. Could someone elaborate the method to prepare sample for TEM analysis...
23 July 2019 5,699 3 View
Can we analyse the morphology of liposomes by Scanning Electron Microscopy (SEM)?
20 July 2019 10,072 4 View
I need the best way to prepare specimens to use with SEM and LOM for my FSW 300x200x3 mm plates of AA6082-T6. I have used Barkers and anodizeing with struers Lectropol-5. Grinding with...
03 July 2019 2,973 3 View
Hello, I'm fabricating nano-pattern through LIL(laser interference lithography). I want to check the height of pattern sample through SEM, since our lab has it. I've tried to check the height of...
02 July 2019 2,895 3 View
Hi everyone, hope you're all okay. So I have some questions/doubts about eds mapping. Just some notes first: I always use the correct accelerating voltage to excite the elements I need, always use...
05 June 2019 3,091 11 View
I need advice from any expert with good SEM knowledge. What could have been the reason for that fault.
31 May 2019 2,595 3 View
Hey folks, hope you're all okay. I have one doubt, I always used to check the videoscope to help improve the bright and contrast in the microscope, but I used to do this in a FEI (Thermofisher)...
18 May 2019 4,248 4 View
Dear Researchers, I did Scanning electron microscopy and have pictures, but now i would like to measure the size of Stomata and trichomes on waxy layer, How can i measure the size?
26 April 2019 3,241 6 View
I want to fit the TCSPC data in origin but the fit with predefined exponential functions of origin is not very good. So please help how to fit it in origin.
15 April 2019 4,427 9 View
I want to do a research about the influence of time on the growth of ZnO nanostructured via hydrothermal, but i don't want to used Si as a substrates or any of substrated. then should i take the...
26 March 2019 2,709 9 View
Hi all, i am new to the SEM imaging system. this is a image of an aluminium sample. any idea how or why these Black shades are formed in SEM secondary electron image?
21 March 2019 6,786 4 View
I'm doing my undergraduate thesis based on medical records and I'm stuck.
11 March 2019 9,617 0 View