Can anyone suggest a trick to get the NO assay standard curve properly?

Hi, I am trying to plot the standard curve for NO scavenging assay with Ascorbic assay. I need this to test the NO scavenging ability of the plant extracts I have prepared. But the results are so...

13 September 2020 1,180 1 View

What can I do if my freeze dried plant extracts wouldn't dissolve in d. water?

Hi, I have prepared freeze-dried plant extracts. Now I want to run antioxidant assays on these plant extracts. But few powders do not dissolve in distilled water. What can I do now? Thank you in...

10 September 2020 8,936 8 View

The condition has length > 1 in ifelse in r?

When the condition has length > 1 in ifelse in r and there are more than 2 statements how to use ifelse? I have a data set wherre I want to categorise people in to categories using sveveral...

04 March 2020 4,811 6 View

When we are using already available records, in a retrospective cohort study the sample size calculation to be done in which formula?

I have started a study of record available at clinic collected years back.So it seems to be a retrospective cohort study.But when calculating sample size I feel like it to be done in normal cross...

12 August 2019 866 3 View

Can machine learning methods to model time-to-event data?

08 May 2019 7,631 2 View

Would you be interested in my sending to Research Gate my bibliography? Many entries, e.g. origin of Aids, published in newspapers are accessible?

Would you be interested in receiving my bibliography of publications? Some publications, such as one on the origin of Aids, are as carefully prepared as in a peer reviewed journal but were offered...

04 June 2018 3,643 3 View

How to convert SNP data in microsoft excel into hapmap?

I have a big data set of SNP in excel format, how can i convert them into hapmap

01 March 2018 2,605 1 View

Contamination in A549 cell culture?

I am culturing A549 cells, I have noticed there are white color particle like things floating on the media. it was visible to the naked eye also. there was no color change in the media or there...

22 February 2018 8,404 5 View

Why there were many floating cell, when THP-1 cells treated with PMA ?

I have done THP-1 cell differentiation into macrophages. I used RPMI as a media. and PMA 30nM concentration. after 24 hours incubation there were many floating cells. what could be the reason? and...

22 January 2018 3,473 3 View

Can someone suggests good molecular markers for population genetics of leafy liverworts?

i'm looking for good molecular markers on populaion genetic studies of leafy liverworts

27 February 2017 6,106 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View