We have crystallized a native protein; it lacks significant homology to any other known structures. We have been trying to crystallize the SeMet-substituted protein, but have difficulties to obtain crystals!
Now we are thinking to soak I3C into our native crystals for phasing. HR protocols says, "Appropriate I3C concentrations in the soaking solution are between 0.05 to 0.5 M." Since we now only have a handful of "precious" candidate crystals to try (it took them 3-6 months to grow), we'd like to know which soaking conditions are most likely to succeed, i.e. sufficient binding but not causing damages to the crystals. Thank you!
Hi,
Yes, I succeeded once:
Article Kti12, a PSTK-like tRNA dependent ATPase essential for tRNA ...
Interestingly, with other several projects it does not work so well, so it is a bit about luck.
Best,
RK
- Badges
- Science method