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Questions related to Scanning Electron Microscopy
I was just wondering, did anyone here observed microvesicles in electron microscopy (TEM) ?? I'd like to discuss-
29 March 2017 6,165 3 View
I have to do SEM analysis of root samples colonized by a selected bacterium. After removal of roots from the pots, how long I can store the samples in 2.5% Glutaraldehyde solution. Is there any...
09 December 2016 5,502 6 View
I make my own FITC/DIG-labelled RNA probes from cDNA using IVT. I would like to clean them up before using them for ISH, preferably using a column. I have already tried them without clean up and...
24 November 2016 6,796 2 View
I need to take some photos of the alveolar bone destruction during periodontitis of BALB/c mouse samples in SEM, but I haven't found a protocol that mentions how to process the sample to clean it...
21 November 2016 7,497 3 View
I do a synthesis with extract plant but I have got problem with the samples, bacause my images´ SEM and TEM are not good
18 November 2016 540 8 View
I've read a few studies on comparisons between SE and BSE mode SEM imaging which show BSE imaging displays less charging. I am also working on block face imaging and the instrument vendors (FEI,...
16 August 2016 6,921 5 View
Say I have slightly different magnification images or say I used two different SEM devices which have different screens so the mag differs. But while preparing the report, I would like to have...
20 July 2016 6,498 3 View
In spite of the fact that I have been in the polymer science business for more than 30 years and have seen and even evaluated several SEM micrographs of polymers, blends and composites, I always...
07 July 2016 4,993 10 View
Dear All, Where can I find an article or information about etchant used for Zr based Bulk Metallic Glasses (both hypo eutectic and eutectic compositions) to be observed in Scanning Electron...
06 June 2016 2,686 1 View
I have fabricated undoped and Cu doped ZnS nanostructures on mica substrates, I got the XRD results which will match the cubic ZnS nanostructures but in this results I got very sharp, very intense...
06 June 2016 9,354 10 View
Or will the heat during the polymerization destroy the fluorophores completely?
06 June 2016 6,337 2 View
How to calculate crystal size by using Scherrer formula? I found a paper in which the author said that he using Scherrer formula calculate the XRD patterns showed in Fig.1 and got the result in...
06 June 2016 3,924 11 View
What should i do to make it clear? My sample is from TiO2
06 June 2016 2,760 1 View
I am trying to see the porosity of cellulose aerogels using SEM
06 June 2016 6,479 3 View
Hi, I have isolated the mitochondria from heart tissue. I wanna do SEM and I don't know how to fix the suspension in slides. Can anyone help me?
05 May 2016 2,456 3 View
Hello researchersBoth of coating and low vacuum can be used to observe non conductive samples by SEM , but which one is better for image quality ?I will appreciate any comparisonregards
05 May 2016 7,344 8 View
i should be achieve EBSD (electron back scatter diffraction) for my current project. but we had not access to it. can i send my specimens to a corporation or institute to do it for me. please guide me
05 May 2016 3,230 5 View
With Thermo Noran system 7 one might compile a synthetic spectrum: Insert manually weight percents of elements, one by one. You may see the synthetic spectrum and identify the elements (same that...
05 May 2016 4,521 0 View
I want to carry on a in-situ experiment in high temperature (about 200℃) by SEM. I want to know whether there will be a severe influence of resolution caused by such a temperature. Dose anyone...
05 May 2016 3,330 5 View
Hello, I am currently interested in the Absorbed current technique in the SEM used as a mapping or imaging system. My samples are BaTiO3 Based MLCC's and i was wondering if anyone has ever used...
05 May 2016 929 3 View
Is it required to adjust Aperture for each magnification in Scanning electron microscope?
05 May 2016 3,619 4 View
Please help me in the characterisation of SEM and FESEM pictures. what we should exactly detremines by seeing these pictures
05 May 2016 2,341 5 View
Recently, I've read chapter 12 of "Mechanical metallurgy" which is written by Dieter. When he mentioned SN curve, he said that there is fatigue limit(or endurance limit) in Ti & Steel's curve....
18 April 2016 6,305 19 View
What would be the optimum dilution of microgels that should be used in the case of FE-SEM sample preparation? I would be using a glass substrate for imaging. I will either spin coat or dry the...
14 April 2016 7,062 4 View