A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Questions related to RNA
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
06 August 2024 1,998 4 View
Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...
06 August 2024 6,250 1 View
How to design VN primer to attach with universal reverse primer
06 August 2024 2,711 3 View
I don't have 12000 g centrifuge. I did 3220g 4 times. It took me some time to add lysate to the column due to debris. According to the protocol, the total volume after adding Elution Buffer and...
06 August 2024 4,749 2 View
The lab I work in has BioRad's Trans-Blot Turbo transfer device, but we'd like to use it with home-made buffers, gels and fibre pads as described in Luisa F Jimenez-Soto's protocol "Trans-Blot®...
06 August 2024 1,378 4 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
05 August 2024 1,535 1 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
05 August 2024 6,584 0 View
Hello everyone, I am currently working on a research project that aims to integrate machine learning techniques into an open source SIEM tool to automate the creation of security use cases from...
05 August 2024 3,252 2 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
04 August 2024 5,908 4 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
03 August 2024 4,235 1 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
02 August 2024 1,449 3 View
The NS1 protein of the tick-borne encephalitis virus potentially has three glycosylation sites. When processing the PNGase F protein on SDS-PAGE, I observe three bands - a fully glycosylated 52...
02 August 2024 3,640 4 View
Hello I am conducting a microstructural analysis of a soil treated with lime. The following sem images are of the untreated s1 and treated soil s3. The untreated soil contains quartz calcite...
02 August 2024 631 0 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
02 August 2024 8,294 2 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
01 August 2024 2,776 1 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
01 August 2024 8,810 13 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
01 August 2024 3,008 6 View
How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?
01 August 2024 5,727 4 View
What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?
01 August 2024 8,362 5 View
I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?
01 August 2024 8,777 1 View
How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?
01 August 2024 9,398 5 View
I am working on natural remedies for de-worming in small ruminant livestocks. Part of my project involves formulation of drench-type product, which I have not much experience in. My experience is...
31 July 2024 10,041 1 View
Dear colleagues, I wonder if combining different research methods, namely literature review, semi-structured interviews and structured questionnaire survey will help to ensure validity and...
31 July 2024 5,208 17 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
30 July 2024 5,545 0 View