A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Questions related to RNA
Problem: Our lab has a DNA synthesizer and sometimes I make in-home synthesized oligonucleotides. From a certain time I have a problem with synthesis of long (>40 nt) oligonucleotides. Short...
22 January 2022 1,094 7 View
Tried "gmx covar" but didn't got the expected results. Please help.
20 January 2022 8,031 6 View
Is there a technique to measure the biofilm formation in the Hungate tubes of autotrophically growing anaerobes?
18 January 2022 2,110 7 View
I'm looking for feedback on the pros and cons of BD's Symphony A5 SE compared to Cytek's Aurora. Things like ease of data acquisition and analysis in addition to customer service, field service...
17 January 2022 8,844 2 View
I want to break the wall of cryptosporidium to obtain DNA directly from the stool sample. Have used many methods but didn't get results. Any suggestions?
15 January 2022 3,661 3 View
Hi colleagues, We injected mice with EdU, fixed tissues with PFA, embedded in OCT, sectioned, and these sections are now stored at -20C. Do you know if this way of processing preserves EdU and...
12 January 2022 170 0 View
I need two proteins co-labeled. Unfortunately, both are are polyclonal antibodies with rabbit as the host. I am trying to look for easy solutions to label one of them with a fluorophore to avoid...
12 January 2022 851 2 View
Dear research community members, If anyone especially those who reside in Thailand knows a sales representative for RPA TwistAmp® basic (TwistDx, UK), please kindly let me know. I will be very...
09 January 2022 5,914 4 View
Hi, Has anyone tried commercial kits for polyA tailing of in vitro transcribed mRNA (e.g. NEB E.coli polymerase kit)? I keep getting inconsistent results, some mRNAs were tailed but some are not,...
09 January 2022 6,443 1 View
Dear all, I am trying to culture human neonatal fibroblast with a keratinocytes together to make an organotypic skin model. Currently, I am working according to the established protocol:...
06 January 2022 7,435 3 View
Hello, I have a problem with the detection of the small RNA ladder on the bioAnalyzer for the quantification of small RNAs. I contacted Agilent, they sent me another ladder. I had the same...
06 January 2022 6,212 0 View
Hi, I'm looking at MaxQuant Evidence output table for my analysis and have following questions: 1. I'm interested in counting the number of peptides (including those not unique). After filtering...
04 January 2022 1,634 2 View
Hello everyone Hope all are doing good. I have a problem with western blot. I'm using Biorad set up for the whole process. I did it 5 times, in the initial stage the protein transfer was not...
30 December 2021 7,764 8 View
hi everyone, I was trying to apply FRED docking between positively charged ligand and a receptor using openeye. the thing is that the command that i used to convert the charged ligand from smi...
28 December 2021 8,924 7 View
I am working on various genotyping that include the digestion of a DNA fragment (obtained by PCR) of approximately 150 base pairs into fragments of approximately 70/80. The starting product on an...
20 December 2021 4,871 2 View
I would like to know what the reason of the shape of this curve in the spectrometer after total RNA isolation using Trizol.?
18 December 2021 10,000 2 View
I need to isolate total RNA from the PBMC after that.
15 December 2021 2,169 6 View
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15 December 2021 9,923 4 View
I used the Mann-Kendall trend test in R as well as XLSTAT for a monthly streamflow data of 30 years (excel file attached) and obtained MK Z-statistic = 0 for one of the monthly time series....
08 December 2021 4,327 5 View
Last time when I did the isolation I got a sequence as shown in the pic below, which is definitely not as required.
06 December 2021 3,603 5 View
I have synthesized nanoparticles by the green method from the double-distilled water extract of the plant as stabilizing and capping agent, nanoparticles confirmed by color change. The...
29 November 2021 9,432 18 View
I have protein spectral counts data obtained after the MS/MS analysis with two conditions: temperature and treatment. What is the best way to calculate the fold change?
27 November 2021 8,177 2 View
Does the difference in the molecular formula of the molecules play into the nature of the results? In other words, does the difference in the molecular formula lead to a difference in the results...
27 November 2021 541 4 View
Dear all, I have been working on antigen presenting experiments in cancer pathogenesis, recently, I am just wondering that is there any tumor-associated antigen that can be chosen to stimulate T...
23 November 2021 9,330 2 View