A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Questions related to RNA
I know that to many of you this might seem like a really stupid question, but I am a beginner with PCR. If I have a DNA sequence (for example, a plasmid) into which I want to insert a small DNA...
24 May 2024 3,637 1 View
We have transfected the plasmid with lipofectamine, the plasmid will eventually move into the nucleus for transcription. Does this plasmid remain in the nucleus during cell replication? if so how...
23 May 2024 5,939 2 View
After PBMC isolation, we are not able to activate and expand T cells.
22 May 2024 6,097 2 View
Can technology enable sustainability and how can technology help reduce the environmental effect of a growing population?
21 May 2024 929 4 View
Good greeting. I want to publish a paper review in a journal specialized in food, health, toxicology, or even environmental sciences, provided that it is indexed in Scopus. I hope you can help...
20 May 2024 4,406 4 View
What does the XRD peak at 43 signify in PVDF-HFP? I want to know which phase I have from this XRD report, but I don't understand why the 43 peak is showing.
19 May 2024 5,052 1 View
If we want to obtain Aerogel from Hydrogel for Oleogel development, does the syneresis of Hydrogel affect the future oil absorption process in Aerogel? Does the volume reduction due to syneresis...
18 May 2024 9,069 1 View
Hello, I'm relatively new to qPCR and have been utilizing a manufacturer's analysis software for my experiments, including one-way ANOVA for statistical analysis. However, I've encountered a...
15 May 2024 3,281 3 View
Hello all. I have recently been trying to isolate total RNA after transfection of miRNA mimics and antisense inhibitors in mammalian cells. However, the RNA isolated through the trizol method...
14 May 2024 4,915 7 View
Which microorganism is responsible for maintaining soil fertility and recycling of matter and role of beneficial microorganisms in soil quality and plant health?
14 May 2024 2,099 0 View
We have been getting this black dots in our cell culture. But it is not affecting pH of media (no color change observed) until 4 or 5 days. Cells are attaining morphology within 24hrs but once the...
13 May 2024 9,262 4 View
ROC curve analysis to detect the best cut-off of the independent variables in the prediction models
13 May 2024 8,390 4 View
The XRD graph are in the file, the composition was mentioned in the graphs but i need determinate the phse for ich peak Thank you very much.
12 May 2024 2,450 2 View
BD Pharmingen™ FITC Mouse Anti-Rat αβ T-Cell Receptor (561672) BD Pharmingen™ APC Mouse Anti-Rat CD4 (550057) BD Horizon™ V450 Rat anti-Mouse CD8a (560469) BD Pharmingen™ PE Mouse Anti-Rat CD45R...
11 May 2024 3,045 0 View
I have designed and assembled a 10kb plasmid containing SbfI and NcoI restriction sites flanking a promoter region, which I am attempting to excise and replace with other promoters. When I do a...
10 May 2024 2,532 1 View
I am isolating the crypt from mouse small intestine the following several modification of EDTA concentration, 3mM (30 min), 5mM (30 min), 20mM (25 min) at 4 degree. For all the EDTA...
10 May 2024 8,975 2 View
Hello I am currently engaged in research focusing on plant genetic transformation. As part of this endeavor, I have designed a comprehensive in silico plasmid cloning approach. The aim is to...
08 May 2024 5,080 3 View
So, I want to extract bone marrow from mice for an experiment, but I don't need all tens of millions of cells that I would get. So I was thinking that I could freeze down the left over cells for...
08 May 2024 7,333 1 View
I am a researcher of Soil science department. 5 months ago I had planted wheat for my research. The research design was split plot design. it has 3 replications, each replication has 2 main plot...
06 May 2024 4,779 3 View
Does anyone know the exact BS concentration of the Zymo DNA methylation gold kit? or is there anyway to measure the BS concentration by titration? Thanks.
06 May 2024 8,819 1 View
What are the effects of Atropine overdose and how is it managed?
05 May 2024 6,132 2 View
The above is the X-ray diffraction diagram from the iron nano sample that I synthesized from green tea extract. Could you please explain the characteristic peak at the 80-degree position?
04 May 2024 4,145 6 View
Is there significant difference between these other element present which highlighted as black? This is from Scanning Electron Microscope-EDS
03 May 2024 6,100 2 View
I have a reference and hit molecules. I need to compare and differentiate the scaffolds present in this hit molecules. my aim is to identify which scaffold that plays a significant role in AD.
02 May 2024 6,535 0 View