Interdendritic phase coarsens during homogenization?

Hello Is it possible for the micro-segregation (non-equilibrium phases) coarsen during the homogenization treatment? There is an obvious reduction in the quantity, but some of the phases remained...

19 September 2020 4,985 3 View

Preparing ethanol standards for HPLC?

I need to prepare ethanol standards for HPLC to quantify the bio-ethanol in my sample of interest. What range of known concentrations would I have to prepare for estimating ethanol concentration...

15 October 2019 8,844 5 View

Quantitative data from a regular FT-IR?

Hi, ATR-FT-IR spectroscopy is being used for the quantification of a particular compound. Is it not possible to run a known quantity of a sample in a regular FT-IR and then compare the peaks...

22 August 2019 9,552 17 View

Can anyone help me with an SDS PAGE's image?

Hi, One of my colleagues has used a pre-cast, commercially available 10% separating gel for his SDS PAGE. Later, while interpreting the results, we observed a broadening of the marker lane...

14 August 2019 8,943 17 View

Grid size in material diffusion simulation - Physical significance?

10 October 2018 8,213 1 View

Chemical mobility from Darken's equation?

19 June 2018 8,585 3 View

Why 002 reflections are absent in spinel oxides (FCC)?

For example, magnetite (Fe3O4) does not contain 002 reflections but contains 004 reflections, though it has FCC structure. Is it because of the structure factor? In my case, I have an oxide that...

29 April 2018 2,053 4 View

What is the difference between graphene and carbon quantum dots??

both quantum dots have the same graphite structure with lattice spacing around 0.34 nm. how can we differentiate?

01 October 2017 3,107 7 View

Can anyone suggest a metal for photovoltaic cell other than gold, silver,and platinum?

To make a circuit in pv cell any metal I want to use as a electrode. For that anyone suggest me a metal other than gold ,silver and platinum.

10 December 2014 2,042 5 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View