A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Questions related to RNA
I have metagenomic data of gut bacteria of tribal population of Himachal Pradesh, can anyone help me regarding analysis of same for writing a research article. we will share authorship for the same.
04 April 2022 4,181 3 View
References or manuals list to analyze 16S rRNA rumen bacteria data
01 April 2022 3,436 2 View
Has anyone tried reverse transcription from a DNA template? How well does it work compared to an RNA template?
29 March 2022 8,682 4 View
Our project is on transcriptomic sequencing of fish brain tissue at different salinity conditions. After isolating the total RNA, we did Bioanalyzer. We got no RIN value and the peak pattern was...
29 March 2022 6,300 1 View
I wanted to do the virome analysis including DNA/RNA bacteriophages and other viruses, microbiome, yeast. What is best approach? Whether we can do it in single reaction or separately, without...
28 March 2022 686 3 View
In what way and when add alumina to our solution of complex in solvent? ( I understood my crystal was acidic with X-ray single crystallograghy and I don't want it. I want a new one with neutral...
27 March 2022 4,671 2 View
Does anyone know how to convert a list of GO-BP terms such as GO_RNA_CATABOLIC_PROCESS to GO-BP IDs such as GO:0006401? Thank you
25 March 2022 4,217 1 View
Hello Researchers I need to measure or calculate milliarbitrary units (mAU) of RNAse? A total of 3.8 mAU of RNAse (Life Technologies) per μg of RNA was added to the formulations Can anybody please...
24 March 2022 465 1 View
I have some newborn mice.(SPF) I want to use antibiotics to clear the intestinal flora of the mother , but I have encountered many difficulties: for example, the mother mouse eats the pups, the...
22 March 2022 6,359 0 View
I am trying to isolate RNA from monocytes, but using the Macherey-Nagel Kit (NucleoSpin RNA Plus) I only seem to get concentrations of 10-20 ng/µL RNA in 20 µL elution volume, which is barely...
18 March 2022 5,404 3 View
Why actin cannot be considered as a suitable reference gene for miRNA differential expression studies? I would like to know what are the best reference genes for miRNA studies under chilling...
17 March 2022 6,981 0 View
According to the protocol, this kit is designed to human blood, but I wonder if I could use it with mouse blood. Could there be a difference in the amount of blood required? Has somebody used it...
16 March 2022 1,913 2 View
Dear colleagues, I am struggling to obtain soil environmental RNA extractions with Zymobiomics RNA Miniprep kit, even while following carefully all steps of this kit designed for this purpose. I...
15 March 2022 3,924 1 View
I am building a custome databse for all viral sequnces so i could run local blast to seperate viral related contigs from host's whole genome sequnce.
15 March 2022 4,509 5 View
Hi everyone, I am struggling with my flow cytometry analysis in FlowJo - I have just downloaded the 30-day free trial on my personal computer. Previously, I have done the analysis in FlowJo at my...
15 March 2022 7,975 1 View
I carried out total RNA non-denaturing agarose gel electrophoresis from 2 human cancer cells. I am not sure what to make of the results as I am finding conflicting statements. Are the first and...
13 March 2022 7,106 3 View
Hello, I am going to work on lncRNA biomarkers in preeclampsia, I wanted to know how stable lncRNAs are in the blood? And how many hours after sampling can I extract lncRNAs from the whole blood?...
10 March 2022 8,458 2 View
Hello! I have been trying to produce IVTs from gBlocks, starting amount is ca. 100/120 ng of DNA. When the reaction is done, I do a DNAse step, purification on column and then go to Nanodrop....
09 March 2022 9,447 3 View
Hi, I have previously used a Qiagen Tissue Lyser to lyse bacteria. Now I'm considering using a vortex (up to 3000 rpm equivalent of 50 Hz). Has anyone swapped between the two, and what is your...
09 March 2022 6,779 0 View
Hello, I am trying to get a metagenomic analysis and found Novogene whose prices are pretty cheap (almost 1/3 of our university core). Does anyone have any experience with this company? about the...
08 March 2022 8,021 6 View
While designing theguide RNA for CRISPR we prefer to use two guide RNA for greater knockout efficiency, So can we design the two guide RNA one from sense and other from antisense strand?
07 March 2022 7,797 2 View
How does Stata calculate the pseudo R squared that it displays after logistic regression and how is it best interpreted?
07 March 2022 5,134 4 View
I am struggling to understand that in an experiment where RT-qPCR will be done to check the expression of a protein, why would we do cDNA synthesis after RNA extraction? I understand it will make...
06 March 2022 3,672 2 View
Hi everyone, I am doing co-IP between two proteins. When I pulldown protein A, I find protein B in the test sample AND the mock sample. The first picture is my first blot, then the second is...
03 March 2022 795 1 View