A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Questions related to RNA
I am trying to load curcumin emulsion in 4% chitosan gel but i don not know how many mg powder is needed ?
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I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...
10 July 2024 4,252 1 View
Can
09 July 2024 6,704 4 View
I am a beginner in this field. While I am reading papers and the understanding is getting better. However can someone explain in easy and simple words. Really thankful !
09 July 2024 8,315 5 View
I have hemostatic nanofibers of hydrophilic nature. While fibrin formation from fibrinogen can be qualitatively visualized using SEM. What assays can be performed on nanofibers to quantify the...
09 July 2024 3,684 1 View
Data Acquisition detected more than 65000 metabolites with area maximum, RT-value and peak-rating. What is the significance of Peak Rating?
08 July 2024 6,623 2 View
Hello, I am Master student under molecular biology. I want to find the Shine-Dalgarno sequence after performing a BLAST search in TargetRNA2. Could you please suggest a web tool to predict the...
08 July 2024 9,573 1 View
i need to ask that when I transduce my HEK293T cells with Lentivirus, how much time will they require for transduction because when I add lentivirus and then change the media after 24 hours, my...
08 July 2024 2,909 1 View
What is the secret behind the success of the researcher named Dariusz Prokopowicz who has a "Research Interest Score" of 11,228 and has 2,620,101 reads in ResearchGate. How was it possible for him...
07 July 2024 2,861 0 View
Hi all, I am looking glass cilinders that have an open flat bottom and top. Preferably with the inner diameter in the range of 2 cm. It's similar to the ones found on multi-electrode arrays for...
07 July 2024 5,826 1 View
Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...
05 July 2024 7,963 2 View
I want to isolate and culture all immune cells from the adipose tissue to isolate EVs. What is the best approach to make sure all cells survive for at least 48h?
04 July 2024 6,479 1 View
bodipy moiety are high polar nature .so its are bottom of TLC PALTE up to 20;80(ethyl acetate;hexane) mixture so its binding in SiO2 SO YIELD is so decreased. how to remove DDQ from reaction mixture?
03 July 2024 8,817 4 View
Hi, I hope you are doing well, I am working to remove DNA from protein sample by using silica hydroxode magnetic beads. I used different binding buffers but I din't get my protein band in initial...
03 July 2024 5,481 4 View
I am trying to eliminate infection from my plates and therefore, gave UV treatment after addition of amino acid (used for better growth). Can you please suggest if UV destroys amino acids or not,...
03 July 2024 2,892 1 View
I am trying to clone a gRNA (23 bases) into the Crispr cas9 pDirect23A vector by using restriction enzyme cloning. I have performed a complete experiment, starting with plasmid isolation,...
03 July 2024 5,420 2 View
I have synthesized MOFs already. However, I need to scale it up to produce 100g of MOFs. During my calculation, I need 15L of DMF just for this synthesis! So I thought, maybe DMF is just used as a...
02 July 2024 1,881 1 View
Hi, All, I am relatively new for exosome isolation. I used an izon column 70nm to isolate exosomes from differentiated neural cells, but the concentrations were pretty low after NTA analysis (I...
02 July 2024 2,528 0 View
Hi everyone, I've been working on differentiating human iPSCs to derive a pure and uniform culture of neural progenitor cells (NPCs). However, I'm encountering a significant issue during the...
02 July 2024 989 1 View
Hi all, I am working on both primary hippocampal mouse neurons and i3Neurons to isolate axonal material. I plate them in microfluidic devices in house made (like any other you can find on the...
02 July 2024 8,047 0 View
The source of classification bias in marker gene metagenome sequencing? a variability in the taxonomy classification of microbial communities when using different primer pairs (e.g. for 16S rDNA)...
30 June 2024 1,005 1 View
How does the water scarcity affect the production of crop and strategies for utilization of poor quality water?
29 June 2024 9,498 6 View
I want to survive in this world without much use of my brain or thinking. How do I still survive in this world without using my brain?
28 June 2024 3,359 1 View
I'm analyzing abundances from various ecological surveys. So data was collected across multiple independent years from three sites within each year. The years fall under two temperature...
27 June 2024 2,772 2 View