A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
4,379 views 5,329 posts
Questions related to RNA
our centrifuge is broken, so I had to centrifuge my cell lysate in RIPA buffer with protease inhibitor for western blot at room temperature not 4*C for 20min. is that OK..?
31 August 2024 3,098 0 View
I should like that my Research gate publications were also on Google Scholar.
30 August 2024 3,965 4 View
Hi everybody, I need to dock a peptide to a glycoprotein. The glycoprotein structure includes sulfate groups and a glycan, which play a crucial role in the peptide's binding. Could you recommend a...
30 August 2024 8,446 0 View
Hi, My lab was doing experiment with a Escherichia strain. It seems it was working well, but after about just 1 month, the negative controls for transformation became positive to Kanamycin and...
28 August 2024 7,321 4 View
Dinoflagellates often incorporate 5-hmU into their genome; does this modification cause issues with PCR, such that we'd need to use uracil-incorporating polymerases, or is a normal high fidelity...
28 August 2024 9,224 1 View
Dear NMR experts, I am looking for a reference with information on the dependence of the chemical shift of typical NMR solvents if varying amounts of acid (e.g. HCl, TFA, MSA...) are present in...
28 August 2024 1,388 2 View
3 different treatment groups (n=12 per group). Several behavioral parameters. There are different timepoints at which the behaviours have been recorded.
27 August 2024 1,197 8 View
Hello everyone, I'm working on DNA cloning and I got some problem for agarose gel electrophoresis after RE digestion. From the internet, I saw the 100ng of DNA will be shown as a sharp and clean...
27 August 2024 9,508 3 View
Help me to find experienced supervisor with excellent lab studying Paleontology and Microbiology
26 August 2024 1,377 3 View
Genetic engineering involves altering the DNA of any organism to achieve desired traits, such as in crops or animals. Gene therapy, on the other hand, specifically targets correcting or replacing...
24 August 2024 3,873 1 View
Kindly support to analyse cognitive behavior in normal adult with free of cost
23 August 2024 2,643 1 View
Dear team I have constructed pFASTBAC1 gene expression construct for expressing viral capsid protein as VLP. Can anyone advise me how to get to get DH10Bac cells or its alternative to transform...
23 August 2024 8,515 1 View
I have a peptide that i cant express in E. coli cells, so i wanted to try with cell free expression kits. I want to know what yield can i expect and since the idea is to use my peptide for NMR,...
22 August 2024 1,307 1 View
Have you ever tried RNA isolation from frozen plasma or serum samples? What concentration range you usually get? Would appreciate if anyone can recommend a protocol?
22 August 2024 5,288 1 View
i am getting a pop up message like " Error dark measurement,value>10%, use basic correction" while doing spectral scan between 200-700 nm with 10 nm interval. both the sample port and reference...
21 August 2024 2,956 1 View
We plan to use BODIPY™ TMR C5-Maleimide to detect free thiols in our protein samples and have designed two approaches. We would appreciate any advice on which method might be more suitable. 1....
21 August 2024 4,386 2 View
TD3 SAC A2C A3C and what else? and which is the latest one?
21 August 2024 1,680 0 View
Recently, we have observed microglia activation. Are there any methods to cool down BV2 cell activation? Would remaking the medium help reduce the activation?
21 August 2024 1,045 0 View
I am currently isolating diazotrophic endophytes from rice cultivars in North East India using nitrogen- free semi- solid media with bromothymol blue as a pH indicator in glass vials. Despite...
21 August 2024 4,880 2 View
We utilize Shimadzu GC-2010 GC-FIDs and using a supelco SP-2560 column. We notice that we experience a significant retention time shift with injection 1 and gradually the retention time will shift...
21 August 2024 9,475 8 View
I have a question about the design of the schema for the cgMLST analysis: why are the genomes with the best quality generally selected for the schema? Which variant is more reliable whether to...
21 August 2024 4,199 0 View
I need review of articles for the definition of humancentric ai and the ways of using it in education
20 August 2024 4,470 6 View
I am currently working on crispr12a with 6x histtaged protein... i am using extraction buffer with with nacl, hepes and protease inhibitor....and in purification iam using nacl, hepesand...
19 August 2024 8,558 5 View
What are the key differences between agarose and polyacrylamide gels in terms of their use in gel electrophoresis?
19 August 2024 3,646 5 View