A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Questions related to RNA
I accidently added five fold more chloroform in the initial steps of RNA isolation. What are the consequences of this? Also, if i add more isopropanol to compensate this effect, how both...
30 June 2022 685 2 View
We are doing 10X single cell sequencing using cryopreserved cells from lymph nodes of lung cancer patients. After thaw, there is around 50% viable cells. Do we need to treat cells with DNAse to...
28 June 2022 3,367 1 View
I am trying to check the effeciency of my segmentation results via CT images. I am looking for a publicly available and annotated dataset of ct images for lung segmetnation. I would be great if...
24 June 2022 9,419 2 View
Hey everyone. I'm using the Sutter p-2000 laser-based micropipette puller to pull needles for zebrafish embryo injection. I used the capillary from sutter: O.D. 1.00mm and I.D. 0.78mm filamented...
23 June 2022 1,441 1 View
Hi! I am using the Y-maze (Spontaneous alternations test) with the Ethovision XT15 software. I have checked the literature and everyone seem to use the percentage of alternations as actual...
22 June 2022 4,153 0 View
Some compounds like, 1-Hexen-4-ol, 1-chloro-3,5-dimethyl 3-Methyl-hepta-1,6-dien-3-ol p-Cymene
22 June 2022 7,137 1 View
It's about RNA viruses metagenome.
21 June 2022 5,262 1 View
hello. I am a student studying MTT assay and Wound healing. Studying MTT and Wound healing, I learned that MTT measures cell viability and Wound healing measures cell proliferation rate and...
18 June 2022 8,874 4 View
Hello, Could someone recommend me an antibody to label macrophages and M1 macrophages for an immunofluorescence on frozen heart sections ? Thank you in advance
16 June 2022 4,321 4 View
What is the best technique to treat regular lung cell lines with exosomes?
16 June 2022 9,369 0 View
I am doing a PCR, and I checked the annealing temperature of my oligos in the NEB Tm calculator. When I selected Q5 polymerase, it said the annealing temperature was 58 degrees celsius, but when I...
13 June 2022 1,661 3 View
I am doing in vitro transcription, my target RNA is 36nt in length. After transcription when I am running it on Denaturing gel, I am not able to see my RNA band under UV shadowing. But when I am...
10 June 2022 4,624 1 View
After purification of our peptibody with MabSelect Protein A chromatography resin (molecular weight ≈60 kDa, pI=8.3), we have two RP-HPLC chromatograms from a biosimilar sample and a standard. It...
10 June 2022 8,131 1 View
I used proteinase K as a protein digestion step before using a Qiagen AllPrep DNA/RNA extraction kit. I have used it before on WT murine heart and skeletal muscle tissues and got good yield....
09 June 2022 8,889 2 View
Hi, Can anyone help me with interpretation of LDH assay? How can I analyze the result?
09 June 2022 8,698 3 View
10-fold dilutions of phage (initial concentration 3E9 PFU/ml) + bacteria (1E5 CFU/ml) were run in triplicate on microplate. Plate was incubated overnite at 37oC, with OD600 measured every 30 min....
09 June 2022 3,436 3 View
I'm going to be isolating RNA (using the Trizol Reagent method) from a bladder cancer cell line and would like to store my isolated RNA in the freezer. I see that in the Trizol protocol, it gives...
08 June 2022 8,197 4 View
I performed a qPCR experiment using QIAGEN's miRCURY LNA RT kit and miRCURY LNA SYBR Green Kits to investigate the miRNA expression levels in some genes, but I could not obtain the desired result...
08 June 2022 1,677 0 View
Hello all, I have always used Trizol reagent to store my samples prior to RNA extraction. I am curious about how to use beta mercaptoethanol as the RNA stabilizer instead of Trizol. What are the...
08 June 2022 9,542 0 View
We know that during S phase cell replicates its genome. Its DNA content gets double. I wanted to know the fate and composition of RNA during interphase
03 June 2022 2,565 2 View
I would like to isolate both DNA and RNA from the same cells (mda-mb-231) after some treatments in culture. I will use different kits for each isolation, I will primarily isolate RNA for QPCR, but...
03 June 2022 8,147 1 View
I use 10ng/ul of RNA for cDNA preparation using iSCRIPT kit....and use 6ul of it in 10ul reaction....i go for nanodrop for cDNA quantification and found 1ug/ul of cDNA...is this possible?
02 June 2022 1,832 3 View
How does fungal infection affect gut microbiota in invasive species ?
02 June 2022 640 0 View
I have purchased a CircLigase II ssDNA Ligase kit and planning my experiments. According to the ligation reaction mixture (shown in attachment) from the Instructions booklet, only 10 pmol of...
31 May 2022 5,308 2 View