A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
1,760 views 4,911 posts
Questions related to RNA
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
02 August 2024 7,652 2 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
01 August 2024 2,012 1 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
01 August 2024 8,109 13 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
01 August 2024 2,378 6 View
How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?
01 August 2024 5,042 4 View
What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?
01 August 2024 7,873 5 View
I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?
01 August 2024 8,386 1 View
How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?
01 August 2024 8,895 5 View
I am working on natural remedies for de-worming in small ruminant livestocks. Part of my project involves formulation of drench-type product, which I have not much experience in. My experience is...
31 July 2024 9,772 1 View
Dear colleagues, I wonder if combining different research methods, namely literature review, semi-structured interviews and structured questionnaire survey will help to ensure validity and...
31 July 2024 5,132 17 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
30 July 2024 4,982 0 View
I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...
29 July 2024 3,537 0 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
29 July 2024 7,961 2 View
The catalytic effect of retained austenite refers to the phenomenon wherein quenching steels with a small amount of retained austenite, when subjected to tempering at a specific temperature...
28 July 2024 8,485 0 View
In order to test different water parameters I need to know how to convert conductivity to Salinity and TDS in ppm or ppt. Give me the reference please.
28 July 2024 2,389 2 View
I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...
26 July 2024 6,495 3 View
What roles do microbial communities play in the environment and roles played by microorganisms in our environment?
26 July 2024 1,952 2 View
How long it takes for arbuscular mycorrhiza to establish and produce benefits under experimental conditions？
26 July 2024 5,188 2 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
26 July 2024 7,487 0 View
It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...
25 July 2024 8,567 2 View
Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...
25 July 2024 2,130 2 View
I am conducting a qualitative-driven approach to mixed-method research. The role of my quantitative data is to corroborate the findings of the qualitative data. Qualitative data has been collected...
25 July 2024 9,767 3 View
How far is the distance with question marks on it? There is a river, where you measure at the point out to the right and there is a low screen. The river is green, what is visible is within the...
25 July 2024 4,364 1 View
Dear all, I gave 116 respondents 18 translated sentences and asked them to indicate their levels of acceptance of these translations on a five-point scale. Some translations result from strategies...
25 July 2024 8,224 5 View