A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Questions related to RNA
I have pdb files of RNA, but need to convert to DNA sequence. How to manually substitute -OH to H using pymol? &
27 April 2022 2,518 4 View
how to overcome cell detachment problem while PBS washing in immunocytochemistry experiment in 96 well plate
27 April 2022 5,105 3 View
Hello everyone, I am having trouble with getting rid of old matrigel fragments when I passage my organoids from an old dome to a new one. I see that some organoids remain stuck in old matrigel...
27 April 2022 3,502 4 View
I am trying to clone HSP90.1 cDNA. For that, I have extracted RNA of Arabidopsis from the leaf. From RNA, cDNA is formed and used for PCR using primer. But I am not getting anything. I have tried...
25 April 2022 6,335 3 View
My RNAseq data and qPCR showed that there is an up-regulation of one lincRNA in the treated cells but when I want to amplify the full length of this lincRNA (1600bp) by RT-PCR, I have no band. Do...
25 April 2022 2,262 3 View
I have used QUAST for visualizing prokaryotic genome assemblies and they worked fine. But for some reason, it is not working for a large eukaryotic mammalian genome assembly. Is there any...
22 April 2022 2,001 2 View
Hi all, I have a problem with VMD wrap and alignment. I simulated a docked RNA and protein complex, but when I wrap the complex following link...
20 April 2022 9,256 2 View
How can I calculate the dn/dc value for RNA in tris and urea buffers?
20 April 2022 5,821 1 View
I am trying to express a short RNA sequences in the nucleus in Drosophila. Are there any UAS-based expression vectors in flies that do not have PAS in the 3'? Or on the other hand, is it possible...
18 April 2022 6,003 3 View
Hello colleagues! I performed RNA extraction for my cells using TRIzol, and when I measured the yield using nanodrop, I got two peaks of RNA. I read that it is because of phenol contamination....
17 April 2022 9,307 3 View
I tried to perform RNA immunoprecipitation assay to reveal RNA partners of the desired protein. Can I store cell extract not the lysate- after cross-linking (1% FA, 10 min RT)- at -80 degrees? Best.
15 April 2022 280 1 View
I want to demonstrate a transcriptional regulation by the coinfiltration with Agrobacterium in N.benthamiana the TF and a promotor sequence. But I don't know which strain of Agro is better for...
15 April 2022 6,347 4 View
Hello Researchers, In most of the articles for RT-PCR of exosomes its suggested to take RNA input as volume (say 6 ul) with addition of spike-ins (for normalization of RT-PCR) and not amount due...
14 April 2022 392 3 View
How is it to differentiate Fecal Proteases if they are from human or bacterial source? There are no specific protease inhibitors for either?
14 April 2022 8,194 3 View
I ran an agarose gel electrophoresis with genomic CHO DNA samples. Some of the samples have been treated with RNase (lanes 2, 4 and 6 - picture attached). There is a band stuck in every well -...
11 April 2022 5,287 3 View
Hi there! I'm performing IHC experiments on PFA-fixed human day 5 embryos (blastocyst stage). After the staining steps, I perform washing in 0.1% Tween-20 in PBS. Although it is usually fine,...
08 April 2022 6,885 1 View
It is to be able to count the number of injuries easily.
08 April 2022 6,846 0 View
We are digesting mouse cortices and trying to extract the RNA from microglia for sequencing. We have gotten the isolation working to get good yield of cells (about 200,000 for both cortices) but...
08 April 2022 7,919 1 View
Hello everyone I am looking for a PAGE gel protocol/recipe for RNA.
07 April 2022 2,310 2 View
I am currently developing an RT-qPCR assay and I'm about to test my primers. I read that I should use different concentrations of template. I have RNA samples with a concentration of 500ng/µl....
07 April 2022 6,367 3 View
Exactly measuring the PD-L1 expression on immunohistologically stained tumor tissue is essential for deciding whether immunotherapy is appropiate or not. The threshold for the indication of immune...
07 April 2022 2,242 0 View
Hi! I am working with the CWP and I need to dialyze it with dialysis membrane of 6000-8000 Da. But I can't understand the term "20 volumes of distilled water. Please help me in finding the...
06 April 2022 6,803 8 View
I'm treating a cell culture with 5-aza-2'-deoxycytidine to evaluate the effect of demethylation in gene expression. I've extracted the RNA and DNA and when measuring the quantity and the quality...
05 April 2022 8,301 1 View
hi everyone, I am new to cell culture and recently I start working with Mia-Paca 2 cell. My question is there any risk working with these cells? as I heard they are highly contiguous. I wear...
05 April 2022 3,741 1 View