A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Questions related to RNA
Any recommendations for a good camera (with underwater housing available) to get detailed photoquadrats (10 x 10 cm) of Dendropoma cristatum encrustations; detailed enough to be able to zoom from...
12 October 2021 9,430 1 View
I am unable to open the raw data files of microarray data...
09 October 2021 1,135 7 View
what should i do for entrapment efficiency test using vivaspin? i,m using vivaspin 500. how long should i take to centrifuge it, and which rpm? and by the end of centrifuge, which part should I...
07 October 2021 5,388 0 View
Hi I am looking for an enzyme with can splice mRNA only at the 5' end with Cap or without Cap generating ~5 nucleotides at the 5' end. Also not degrading the rest of the mRNA. I am using RNase H...
30 September 2021 7,215 1 View
I used SaliLab Model Evaluation Server to evaluate the model generated in homology modelling. I got a predicted RMSD score of 3.830 Å, but I don't understand how to interpret the number, whether...
26 September 2021 8,069 4 View
Currently, I'm working with the RAW 264.7 cell line. We used to use Trypsin-EDTA to remove cells from the surface of other cell lines, however, ATCC recommended using a cell scraper instead of...
25 September 2021 3,799 2 View
I would like to know the cDNA quality before proceeding to PCR. I learnt the use of Nano drop may not be helpful as the cDNA synthesis makes use of RNA and mixture of dNTPs, random hexamers and/or...
23 September 2021 5,814 3 View
Hi, I am trying to isolate MSCs from peripheral blood for the generation of iPSCs, wich will be used for neural differentiation. I would like to ask if anyone has succeed in isolating MSCs from...
20 September 2021 2,360 5 View
Currently I am performing FISH in situ hybridization of human telomeres with PNA probes. However, one thing happened during my experiment that there is too much background noise when I observe it...
09 September 2021 694 3 View
For the cell count of bacteria, we want to know how it differs from each other . which is more preferable?
08 September 2021 9,857 4 View
Hello I am trying to extract RNA from the bone I have tried the Trizol method and I got a high yield of RNA with slightly low 260/280 ratio. when I measure the RNA integrity I got very bad...
06 September 2021 7,959 1 View
Hi everyone, I am reading up on gene editing with CRISPR/Cas9. I wanted to understand how one assesses the gene editing efficiency of an experiment. Correct me if I am wrong, I understand the...
26 August 2021 9,669 6 View
Hello everyone: I am planning to start a Master degree in Bioinformatics. My original filed of science is Biotechnology Engineering. Could you recommend some basic references in the field of...
26 August 2021 3,851 7 View
Dear all, I am currently working on a reverse-transcription protocol (otal RNA extracted from CHO cells, then reverse-transcribed to be used in qPCR) with QuantiNova Reverse Transcription kit...
20 August 2021 6,031 2 View
I use 1-2g fresh surgical samples of endometrial cancer patients and temporarily save it in MACS® Tissue Storage Solution(Miltenyi company), then I digest the tissue in 10%FBS 1640 medium with 1...
19 August 2021 3,572 0 View
Hi everyone, I performed a miniprep to verify if a correct construct was made. The overall purpose of my project is to put RFP gene into puc19. I did Nanodrop to measure DNA concentration and the...
15 August 2021 8,619 2 View
I would like to know if it is possible to dock a ligand and RNA in DOCK6? The few tutorials I have seen for DOCK6 are based off docking a ligand and a protein. Additionally, what would be the...
11 August 2021 3,637 2 View
I am using a Zymo RNA shield reagent to store the samples. The extraction kit used is the RNeasy Kit from Qiagen.
09 August 2021 4,684 3 View
Hi all I have a question about selection of subsets of genes for ontology analysis. In some studies, only either up-regulated or down-regulated genes are selected for ontology analysis, whereas...
08 August 2021 6,566 4 View
Hi! A special primer has to be used with only 19 bp poly dT (Tm 44.9) instead of 30 bp in original study (Tm 55.2). Will this induce failure of mRNA capture? Will this cause RNA detachment in...
07 August 2021 5,500 3 View
I need to extract total RNA from rat different tissues with trizol to do the qRT-PCR. I have tried twice. but, I could not get the high-quality RNA, this is my protocol, anyone can help me check...
05 August 2021 9,439 2 View
I have a multimodal/polydispersed sample of copper nanoparticles. Batch DLS gives scattered results probably because larger particles scatter more light and the DLS algorithm counts the smaller...
05 August 2021 3,057 4 View
I am using glass milk (silica) to purify RNA. However the protocol I am following uses acidic KI. Does the chaotropic agent has to be acid to allow RNA binding to the silica?
04 August 2021 3,903 1 View
I'm trying to do osteogenic differentiation of human dental pulp stem cells. Osteogenic differentiation culture media was constituted of DMEM, 10% FBS, 50 µM ascorbate acid, 10 mM β-glycerol...
04 August 2021 2,113 4 View