A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Questions related to RNA
Hello! I have been having the following blot after western blot transfer, and I am not sure what the problem is... I make a resolving and stacking gel following the TGX fastest 12% acrylamide kit...
11 April 2024 9,647 1 View
I've received antibodies diluted in P.B.S. instead of 5% BSA in 1X T.B.S. Will this dilution work for IHC or I've to discard the dilution ?
11 April 2024 6,834 1 View
Red bone marrow contains blood stem cells that can become red blood cells, white blood cells, or platelets. Yellow bone marrow is made mostly of fat and contains stem cells that can become...
11 April 2024 5,989 1 View
I did PCR of 6 amplicons and when I did agarose gel electrophoresis, they all appear consistently bigger than they should be (e.g. the amplicon should be 4000 bp, but is around band of 5000 bp...
10 April 2024 4,437 5 View
Which software to compare two genomes
10 April 2024 4,551 2 View
Hey everyone! I'm currently delving into computational protein engineering and protein docking and would love to explore some friendly and accessible literature and methods books on the subject....
09 April 2024 1,606 0 View
I want to conjugate my antibody with Dylight-NHS ester and corboxylated fluorescent PS beads. How do I check whether both Dylight-NHS ester and corboxylated fluorescent PS beads coupled with my...
09 April 2024 6,924 2 View
Hello everyone, I am supervising a master thesis student whose goal is to compare different lysis methods for recombinant protein purification. We are working with E. coli pellet and he is...
09 April 2024 6,212 7 View
Can we add midori green directly while preparing polyacrylamide gels? or should only be done as a post staining after running the PAGE gel?
09 April 2024 2,177 3 View
I need to develop an enzyme assay to evaluate the degradation of gluten using proteases. Previously, I conducted tests with finished products, which was straightforward. However, I now have to...
08 April 2024 1,859 3 View
what strategies can be employed to mitigate these effects?
08 April 2024 4,891 1 View
I'm developing a lateral flow assay at present and I've had trouble with my antibody-gold conjugate not moving off my conjugate pad. When I run test solutions through it (PBS, PBS + 1% Tween20,...
07 April 2024 6,842 3 View
Explain the concept of complexity theory Discuss the link between resilience and complexity theory Explain how these relate to public policy illustrate your discussion with appropriate example
06 April 2024 8,865 1 View
MTT assay
04 April 2024 1,683 3 View
How do these abnormalities impact male fertility?
03 April 2024 895 2 View
I would like to measure the oxygen consumption rate directly from tissue in the seahorse XFe96 analyzer with the Seahorse XFe96 Spheroid FluxPak (Part Number:102905-100) but i need a specific glue...
03 April 2024 8,697 1 View
My primers are designed to yield 1400 bp fragment. But on the gel after PCR, I am getting sharp band at 1000 bp and faint band at 1500 bp. What are the troubleshoot options that I can apply to get...
03 April 2024 589 0 View
Why we do not feed Daphnia during the 48 hours. Will that not affect them?
02 April 2024 648 0 View
I am looking for help to optimize a nested PCR from Long Range (LR) PCR (DNA as template). The LR PCR product looks spesific on agarose gel. We have tried various dilutions of this product as...
02 April 2024 6,810 5 View
I am preparing a drug for oral gavage which needs to be dissolved in 0.5% methylcellulose. The drug powder remains as clump and refuses to go into solution. Any tips on this?
02 April 2024 4,825 3 View
I’m trying to induce my drug of different concentrations into my cell line. I’m working with HepG2 cell line
02 April 2024 8,330 4 View
Hi, I am growing Caco2 cells in 24 Transwell cell inserts. I am planning to induce gut leakage by LPS and want to study gene expressions. My concern is to isolate the RNA from transwell inserts...
02 April 2024 5,734 3 View
I am trying to find out what will be the effects of microplastics in cells and for that, I will use a fluorescent microplastic to act as a stressor and then measure ROS by fluorescence. I will...
02 April 2024 250 1 View
Hi all, I have just started to learn about bioinformatics and I need help with it. I have enriched some microbes from wastewater anaerobic sludge and sent them for 16S rRNA sequencing. Based...
30 March 2024 5,246 4 View