A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Questions related to RNA
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
22 July 2024 5,398 4 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
22 July 2024 8,807 1 View
Hello! I wonder how I can add to the list of journals, a journal published in Greece .
22 July 2024 7,635 1 View
I want to assign a DOI to works published on Research Gate. I thought Research Gate assigned them, but I don't see how.
21 July 2024 1,583 0 View
One has to understand about the enhancement procedures and treatment systems I can list around 12 procedures which are tried and tested and are proven so let us see how many in total the experts...
21 July 2024 3,835 2 View
Dear Pe
21 July 2024 9,764 6 View
Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...
21 July 2024 5,735 2 View
How to assign allele numbers and sequence type after amplifying housekeeping genes by PCR for genotyping of S. pneumoniae strains by MLST using bioedit ?
20 July 2024 5,343 2 View
Experts are welcome to share their experience country-wise so that we can make a nice document listing all the country-wise experiences. ? How does the digestate compare to other organic...
19 July 2024 5,517 3 View
I'm currently working with human pancreatic organoids and I am wondering to remove the dead cells and debris from health organoids, any suggestions would be appreciated!
18 July 2024 8,547 1 View
Hello, I'm trying to create parameter and coordinate files for a drug (PRG-A01) found on the following page: http://www.probechem.com/products_PRG-A01.html I used the guide made by Michael...
18 July 2024 3,761 3 View
I am designing research related to atherosclerosis in vitro, but unfortunately the cell lines currently available in our laboratory are only 3T3-L1 and H9C2, Does anyone can suggest me what...
18 July 2024 6,436 1 View
Hello, how can I do the statistics associated with genotypes and alleles as in the table? thank you very much for your attention.
18 July 2024 7,667 2 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
17 July 2024 9,257 10 View
Hi All I am looking to get your opinions, I have perfomed liposome mediated DNA transfer into bacteria, the DNA is a plasmid with msfGFP and when I induce the plasmid I see the fluorescence, and...
17 July 2024 4,779 4 View
i need to know the required time
16 July 2024 5,063 2 View
I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...
16 July 2024 6,158 0 View
Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?
16 July 2024 2,186 3 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
16 July 2024 6,855 0 View
Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...
16 July 2024 5,075 1 View
I am working on a dataset of MHC-I and II alleles from a bird species sequenced with Illumina. We were not able to assign alleles to loci through MHC-typer as we were over the limit of 150 alleles...
16 July 2024 295 1 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
16 July 2024 1,724 3 View
Dear all, I'm going to radiolabel antibodies. So first of all, I'll remove excipients using the centrifugation method with amicon. Once I'm done, how do I calculate/measure the concentration of...
15 July 2024 3,169 3 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
15 July 2024 8,725 3 View