How can we now if the promotor in the vector is well?

03 August 2024 1 4K Report

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil.

The bacteria that I want to use for cloning haven't been identified and not be sequenced, previously. How can I undrestand which promotor is the best and can be clone without any problem?

Ananth Krishna Narayanan

Hi Bahare Khoshkholgh

There are many ways to approach this problem depending on the resources available to you. But I think you should first identify the organism you are working with. There are molecular markers which can help you do this like ITS or rDNA.

Once you have confirmed it is a Pseudomonas sp. you can search the literature for promoters and even expression plasmids.

Hope this helps

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